標(biāo)題: Titlebook: Amino Acid Analysis; Methods and Protocol Michail A. Alterman Book 2019Latest edition Springer Science+Business Media, LLC, part of Springe [打印本頁] 作者: GERD847 時(shí)間: 2025-3-21 17:18
書目名稱Amino Acid Analysis影響因子(影響力)
書目名稱Amino Acid Analysis影響因子(影響力)學(xué)科排名
書目名稱Amino Acid Analysis網(wǎng)絡(luò)公開度
書目名稱Amino Acid Analysis網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Amino Acid Analysis被引頻次
書目名稱Amino Acid Analysis被引頻次學(xué)科排名
書目名稱Amino Acid Analysis年度引用
書目名稱Amino Acid Analysis年度引用學(xué)科排名
書目名稱Amino Acid Analysis讀者反饋
書目名稱Amino Acid Analysis讀者反饋學(xué)科排名
作者: 走調(diào) 時(shí)間: 2025-3-21 21:11
https://doi.org/10.1007/978-3-7091-7711-2n and .-tartaric acid have been described as chiral selector either as impregnating reagent in the stationary phase or as an additive in the mobile phase for direct enantioseparation by TLC. Application of the homemade TLC plates has also been described; the methods are successful in obtaining the native enantiomer as well.作者: 射手座 時(shí)間: 2025-3-22 02:34 作者: 單調(diào)女 時(shí)間: 2025-3-22 05:49 作者: amyloid 時(shí)間: 2025-3-22 09:29 作者: 官僚統(tǒng)治 時(shí)間: 2025-3-22 15:39 作者: 抓住他投降 時(shí)間: 2025-3-22 18:48 作者: HAUNT 時(shí)間: 2025-3-23 01:10
,Der Nachweis der Antik?rperproduktion,ine, phenylalanine, proline, and valine) were in the range of 6–80 and 20–200?fmol, respectively. As little as 25?pmol of peptide or protein hydrolysate is sufficient for determining absolute content.作者: kyphoplasty 時(shí)間: 2025-3-23 04:06 作者: Infantry 時(shí)間: 2025-3-23 08:47
Forschungen zur Europ?ischen Integrationimple protocol for in situ derivatization of amino acids with heptafluorobutyl chloroformate (HFBCF) followed by subsequent chiral as well as nonchiral GC/MS (mass spectrometric) analysis on a respective nonpolar fused silica and an enantioselective Chirasil-Val capillary column.作者: 嗎啡 時(shí)間: 2025-3-23 13:22
Forschungen zur Europ?ischen Integrationsimultaneous analysis of 18 .-amino acids with high sensitivity and reproducibility. Additionally, this chapter also focuses on the application of the method to real samples for the quantification of targeted amino acids.作者: LINE 時(shí)間: 2025-3-23 15:20 作者: 單調(diào)性 時(shí)間: 2025-3-23 19:15
Heptafluorobutyl Chloroformate-Based Sample Preparation Protocol for Nonchiral and Chiral Amino Aciimple protocol for in situ derivatization of amino acids with heptafluorobutyl chloroformate (HFBCF) followed by subsequent chiral as well as nonchiral GC/MS (mass spectrometric) analysis on a respective nonpolar fused silica and an enantioselective Chirasil-Val capillary column.作者: Malaise 時(shí)間: 2025-3-23 23:40 作者: 灌溉 時(shí)間: 2025-3-24 06:11 作者: ELUDE 時(shí)間: 2025-3-24 07:48 作者: 捏造 時(shí)間: 2025-3-24 12:43 作者: asthma 時(shí)間: 2025-3-24 15:34
Amino Acid Analysis by Hydrophilic Interaction Chromatography Coupled with Isotope Dilution Mass Spine, phenylalanine, proline, and valine) were in the range of 6–80 and 20–200?fmol, respectively. As little as 25?pmol of peptide or protein hydrolysate is sufficient for determining absolute content.作者: CLIFF 時(shí)間: 2025-3-24 23:05
1064-3745 ation advice from the expertsAmino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method; another new aspect is miniatur作者: 信條 時(shí)間: 2025-3-25 01:15
https://doi.org/10.1007/978-3-7091-7730-3recombinant proteins. The protocol is based on a microwave-assisted acidic hydrolysis of the target protein followed by high-resolution mass spectrometry (HRMS) analysis of the hydrolytic products. Absolute quantification is obtained by adding controlled amounts of labeled amino acids that serve as standards.作者: MAZE 時(shí)間: 2025-3-25 05:26 作者: Celiac-Plexus 時(shí)間: 2025-3-25 09:13 作者: 僵硬 時(shí)間: 2025-3-25 14:01 作者: 紳士 時(shí)間: 2025-3-25 17:56
https://doi.org/10.1007/978-3-7091-7711-2updated description of the chromatographic setup and selected reaction monitoring (SRM) transitions. Then, we describe sample processing for serum, plasma, urine, cerebral spinal fluid, and dried blood spots. Most importantly, we outline a singular quantitative design for efficient data analysis of 作者: Allege 時(shí)間: 2025-3-25 20:15
https://doi.org/10.1007/978-3-7091-7711-2. The alkaline nature of the elution medium ensures that the AA cations are released prior to derivatization. The latter involves production of chloroformate derivatives of both the amino and carboxylic acid groups. With experience, six plasma samples can be so processed within 12?min. The shortest 作者: 吼叫 時(shí)間: 2025-3-26 01:31
,Der Nachweis der Antik?rperproduktion,5?°C) in an autosampler. Their derivatives are immediately injected into the HPLC column without the need for extraction. Trp metabolites that cannot react with OPA but are fluorescent can be detected by setting the excitation and emission wavelengths of the fluorescence detector in another detectio作者: 不適當(dāng) 時(shí)間: 2025-3-26 07:53
,Der Nachweis der Antik?rperproduktion,sis method used at NIST for assigning concentration values in SRM deliver the highest accuracy and precision possible. Therefore, we have developed an amino acid analysis method that uses isotope dilution LC-MS/MS—the analytical technique routinely used at NIST to certify analyte concentrations in S作者: Fortuitous 時(shí)間: 2025-3-26 10:02 作者: 刺激 時(shí)間: 2025-3-26 16:24 作者: 擴(kuò)音器 時(shí)間: 2025-3-26 17:09 作者: 滋養(yǎng) 時(shí)間: 2025-3-26 23:43 作者: Folklore 時(shí)間: 2025-3-27 03:43 作者: 乳白光 時(shí)間: 2025-3-27 07:29
Amino Acid Analysis by Means of MALDI TOF Mass Spectrometry or MALDI TOF/TOF Tandem Mass Spectrometique is capable of distinguishing isobaric and isomeric compounds. Both methods do not require amino acid derivatization or chromatographic separation, and the data acquisition time is decreased to several seconds for a single sample.作者: ungainly 時(shí)間: 2025-3-27 13:20 作者: CUB 時(shí)間: 2025-3-27 17:35
The EZ:Faast Family of Amino Acid Analysis Kits: Application of the GC-FID Kit for Rapid Determinat. The alkaline nature of the elution medium ensures that the AA cations are released prior to derivatization. The latter involves production of chloroformate derivatives of both the amino and carboxylic acid groups. With experience, six plasma samples can be so processed within 12?min. The shortest 作者: 搖曳 時(shí)間: 2025-3-27 18:25 作者: aerial 時(shí)間: 2025-3-27 22:06
Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry for Quantitative Amino Acid Analysisis method used at NIST for assigning concentration values in SRM deliver the highest accuracy and precision possible. Therefore, we have developed an amino acid analysis method that uses isotope dilution LC-MS/MS—the analytical technique routinely used at NIST to certify analyte concentrations in S作者: canonical 時(shí)間: 2025-3-28 04:08
Quantification of Underivatized Amino Acids on Dry Blood Spot, Plasma, and Urine by HPLC-ESI-MS/MS,o 10?min with very good reproducibility of retention times and a better separation of the metabolites and their isomers..The omission of the derivatization step, enabled?to achieve some important advantages: fast and simple sample preparation, exclusion of artifacts, and interferences. The use of th作者: 散開 時(shí)間: 2025-3-28 06:33 作者: machination 時(shí)間: 2025-3-28 14:28 作者: 調(diào)整 時(shí)間: 2025-3-28 15:51
Ultrasensitive Quantification of Recombinant Proteins Using AAA-MS, be purified and their quantity and/or concentration precisely determined. In this chapter, we describe a protocol for the quantification of purified recombinant proteins. The protocol is based on a microwave-assisted acidic hydrolysis of the target protein followed by high-resolution mass spectrome作者: Criteria 時(shí)間: 2025-3-28 19:19 作者: 盲信者 時(shí)間: 2025-3-28 23:22
Amino Acid Analysis by Means of MALDI TOF Mass Spectrometry or MALDI TOF/TOF Tandem Mass Spectrometrometry (MS). First protocol describes a MALDI TOF MS-based method for a routine simultaneous qualitative and quantitative analysis of free amino acids and protein hydrolysates. Linear responses between the amino acid concentration and the peak intensity ratio of corresponding amino acid to internal作者: TAIN 時(shí)間: 2025-3-29 06:27
Multiple Reaction Monitoring for the Accurate Quantification of Amino Acids: Using Hydroxyproline tadopted for the analysis of small molecules especially within the pharmaceutical industry. It can also be applied to the analysis of peptides and proteins and to measurement of the basic building blocks of proteins, amino acids. Here we describe the application of MRM mass spectrometry to the measur作者: impale 時(shí)間: 2025-3-29 09:18
Analysis of Hydroxyproline in Collagen Hydrolysates, in all collagen types of all species. In both Xaa- and Yaa-positions, Pro is the most common residue, often oxidized to 4-Hyp in the Yaa- and rarely to 3-Hyp in the Xaa-positions. Here we describe the qualitative and quantitative analysis of 3- and 4-Hyp-isomers by separating the free imino acids e作者: NAUT 時(shí)間: 2025-3-29 14:38 作者: BUMP 時(shí)間: 2025-3-29 18:50 作者: 無關(guān)緊要 時(shí)間: 2025-3-29 21:52 作者: 退出可食用 時(shí)間: 2025-3-30 01:10 作者: Angioplasty 時(shí)間: 2025-3-30 05:19
The EZ:Faast Family of Amino Acid Analysis Kits: Application of the GC-FID Kit for Rapid Determinatfamily of kits. Three kits are available: (1) GC-FID or -NPD, (2) GC-MS, and (3) LC-MS. The two GC kits can determine 32 AA, whereas the LC-MS can determine 5 additional AA. All three kits, however, share the same experimental procedure up to and including the preparation of derivatized AA. The meth作者: Fecundity 時(shí)間: 2025-3-30 09:16 作者: Negotiate 時(shí)間: 2025-3-30 14:51 作者: Accessible 時(shí)間: 2025-3-30 16:40 作者: Acquired 時(shí)間: 2025-3-30 23:28
Amino Acid Analysis in Physiological Samples by GC-MS with Propyl Chloroformate Derivatization and ol derivatization and gas chromatography-quadrupole mass spectrometry (GC-qMS) analysis in a single-ion monitoring mode. Derivatization is carried out directly in the aqueous samples, thereby allowing automation of the entire procedure, including addition of reagents, extraction, and injection into 作者: peak-flow 時(shí)間: 2025-3-31 01:57
,Combination of an AccQ?Tag-Ultra-Performance Liquid Chromatographic Method with Tandem Mass Spectrods in complex samples face intrinsic challenges such as insufficient sensitivity, selectivity, and throughput. This chapter describes a protocol that makes use of AccQ?Tag chemical derivatization combined with the exceptional chromatographic resolution of ultra-performance liquid chromatography (UPL作者: Ingest 時(shí)間: 2025-3-31 09:03 作者: inquisitive 時(shí)間: 2025-3-31 11:31
Analysis and Enantioseparation of Amino Acids by Liquid Chromatography, i.e., HPLC and TLC. A researcher who wants to perform amino acid (AA) analysis or separate enantiomers of AAs by HPLC or TLC can follow the method. Figures included represent the actual experiments..Synthesis and application of chiral derivatizing reagents (CDRs) based on cyanuric chloride (CC) and作者: 沒有希望 時(shí)間: 2025-3-31 13:31 作者: 沒收 時(shí)間: 2025-3-31 18:24
High-Throughput LC-MS/MS Method for Chiral Amino Acid Analysis Without Derivatization,iological functions that differ from .-amino acid. Most chiral amino acid separation techniques require complicated derivatization procedures in order to achieve the desirable chromatographic behavior and detectability. This chapter describes a highly sensitive analytical method for the enantiosepar作者: goodwill 時(shí)間: 2025-4-1 01:08
Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Speaddition of isotopically labeled standard amino acids and analysis by mass spectrometry for accurate and sensitive protein quantitation. Quantitation of less than 10?fmol of protein standards with errors below 10% has been demonstrated using this method.作者: FIS 時(shí)間: 2025-4-1 05:55 作者: condescend 時(shí)間: 2025-4-1 06:00
https://doi.org/10.1007/978-3-7091-7730-3addition of isotopically labeled standard amino acids and analysis by mass spectrometry for accurate and sensitive protein quantitation. Quantitation of less than 10?fmol of protein standards with errors below 10% has been demonstrated using this method.作者: Mangle 時(shí)間: 2025-4-1 13:19
https://doi.org/10.1007/978-3-7091-7730-3 be purified and their quantity and/or concentration precisely determined. In this chapter, we describe a protocol for the quantification of purified recombinant proteins. The protocol is based on a microwave-assisted acidic hydrolysis of the target protein followed by high-resolution mass spectrome
