標(biāo)題: Titlebook: Alternative Splicing; Methods and Protocol Peter Scheiffele,Oriane Mauger Book 2022 The Editor(s) (if applicable) and The Author(s), under [打印本頁] 作者: ACORN 時間: 2025-3-21 17:32
書目名稱Alternative Splicing影響因子(影響力)
書目名稱Alternative Splicing影響因子(影響力)學(xué)科排名
書目名稱Alternative Splicing網(wǎng)絡(luò)公開度
書目名稱Alternative Splicing網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Alternative Splicing被引頻次
書目名稱Alternative Splicing被引頻次學(xué)科排名
書目名稱Alternative Splicing年度引用
書目名稱Alternative Splicing年度引用學(xué)科排名
書目名稱Alternative Splicing讀者反饋
書目名稱Alternative Splicing讀者反饋學(xué)科排名
作者: 成份 時間: 2025-3-21 22:11 作者: 古老 時間: 2025-3-22 04:17
Alireza Bahadori,Scott T. Smithe fluorescent probes that are highly specific to their RNA target. To facilitate probe design, we have created anglerFISH, a user-friendly command-line based pipeline. In this chapter, we present how to perform a smFISH experiment using user-designed and labeled probes.作者: pericardium 時間: 2025-3-22 04:50
Identification and Quantification of Microexons Using Bulk and Single-Cell RNA-Seq Data,xons of length up to 30?nt, require specialized computational workflows. Here, we describe MicroExonator, a reproducible computational workflow for microexon splicing analysis using bulk or single-cell RNA-seq data.作者: 蝕刻 時間: 2025-3-22 11:04 作者: LAY 時間: 2025-3-22 14:47
R. G. Mirimanov,N. E. Sirotina,V. I. Neymanregulatory conservation among species (.), and (d) help with the biological interpretation of the results, and, ultimately, with the identification of interesting AS events to design wet-lab experiments (. and .).作者: 寬容 時間: 2025-3-22 18:44 作者: Customary 時間: 2025-3-22 22:59
https://doi.org/10.1007/978-1-4419-9713-5ynthetic splicing factors using solution state NMR spectroscopy. This approach allowed us to validate direct interactions between splicing regulators and U1 snRNP and could also be useful for the screening of small molecules acting on splicing regulation.作者: Plaque 時間: 2025-3-23 02:05 作者: 群居動物 時間: 2025-3-23 08:33
Computational Analysis of Alternative Splicing Using , and the , Framework,regulatory conservation among species (.), and (d) help with the biological interpretation of the results, and, ultimately, with the identification of interesting AS events to design wet-lab experiments (. and .).作者: 緯度 時間: 2025-3-23 11:03
,BaseScope? Approach to Visualize Alternative Splice Variants in Tissue, relative to e37b-. and both exons share 60% of their sequence. By using BaseScope?, we were able to discover that e37a-. is expressed in excitatory pyramidal neurons of hippocampus and cortex, as well as motor neurons of the ventral horn of the spinal cord.作者: 使混合 時間: 2025-3-23 14:14 作者: refine 時間: 2025-3-23 22:04 作者: 結(jié)束 時間: 2025-3-24 00:50 作者: 使成波狀 時間: 2025-3-24 06:05
Book 2022tion of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution,作者: stroke 時間: 2025-3-24 09:32 作者: granite 時間: 2025-3-24 11:32
https://doi.org/10.1007/978-94-017-1251-4characterization of RNA isoforms and led to the perpetual incrementation of gene expression diversity. Here, we describe a high throughput approach to assess in-depth the splicing regulation of target gene(s) using the third-generation sequencing (TGS) technologies.作者: evaculate 時間: 2025-3-24 18:31
https://doi.org/10.1007/978-1-4419-9713-5 reporter system that enables the visualization of alternative splicing patterns by microscopy at single-cell resolution in live animals. We present this reporter system in the context of the model nematode ..作者: 古文字學(xué) 時間: 2025-3-24 23:05 作者: 身體萌芽 時間: 2025-3-25 01:36 作者: RUPT 時間: 2025-3-25 06:54
Analysis of Splicing Regulation by Third-Generation Sequencing,characterization of RNA isoforms and led to the perpetual incrementation of gene expression diversity. Here, we describe a high throughput approach to assess in-depth the splicing regulation of target gene(s) using the third-generation sequencing (TGS) technologies.作者: BILIO 時間: 2025-3-25 08:58
Two-Color Fluorescent Reporters for Analysis of Alternative Splicing, reporter system that enables the visualization of alternative splicing patterns by microscopy at single-cell resolution in live animals. We present this reporter system in the context of the model nematode ..作者: Nausea 時間: 2025-3-25 13:26 作者: 強有力 時間: 2025-3-25 16:13 作者: OMIT 時間: 2025-3-25 22:44
The Dictionary of Drugs: Chemical Data on addressing the specificity of isoform quantification?and describe a simple sensitive method. Quantitative measurement of alternatively?spliced RNA isoforms can be used to differentiate splicing regulation from transcriptional control and isoform-specific RNA decay regulation.作者: BANAL 時間: 2025-3-26 02:25
https://doi.org/10.1007/978-3-642-98090-9uided annotation of novel transcripts. A key part of our protocol is the R package . that rapidly matches custom-assembled transcripts to their likely host genes, deduces the sequence and domain structure of novel protein products, and predicts sensitivity of newly identified RNA isoforms to nonsense-mediated decay.作者: 歌曲 時間: 2025-3-26 05:56
Part III Food Definitions and Formulationsenome-wide CRISPR screening. We have successfully used this approach to identify novel regulator of IR of the MAT2A transcript and propose that similar screens will be broadly applicable for the identification of novel factors that control IR of specific transcripts.作者: 柔軟 時間: 2025-3-26 12:27
https://doi.org/10.1007/978-1-4419-9713-5 (MS2CP) with high affinity and specificity and by doing so, the POI is tethered to the reporter RNA. Here, we describe how with the help of this assay the human cytoplasmic poly(A) binding protein is recruited to a mini-μ RNA reporter, thereby influencing the stability of the reporter transcript.作者: WAIL 時間: 2025-3-26 14:11
Alternative Splicing in Human Biology and Disease,patterns can cause or contribute to a wide range of diseases. In this introductory chapter, we outline the mechanisms that govern alternative pre-mRNA splicing and its regulation and discuss how dysregulated splicing contributes to human diseases affecting the motor system and the brain.作者: 觀點 時間: 2025-3-26 17:05
,Quantitative Measurement of Alternatively Spliced RNA Isoform?Levels, on addressing the specificity of isoform quantification?and describe a simple sensitive method. Quantitative measurement of alternatively?spliced RNA isoforms can be used to differentiate splicing regulation from transcriptional control and isoform-specific RNA decay regulation.作者: Ostrich 時間: 2025-3-26 21:16 作者: Criteria 時間: 2025-3-27 04:13 作者: 軟弱 時間: 2025-3-27 08:47
Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins, (MS2CP) with high affinity and specificity and by doing so, the POI is tethered to the reporter RNA. Here, we describe how with the help of this assay the human cytoplasmic poly(A) binding protein is recruited to a mini-μ RNA reporter, thereby influencing the stability of the reporter transcript.作者: 思想靈活 時間: 2025-3-27 12:07 作者: Infinitesimal 時間: 2025-3-27 15:49 作者: heckle 時間: 2025-3-27 21:37
,An Optimized Protocol for the Mapping of Cell Type–Specific Ribosome-Associated Transcript Isoformsion and cellular functions. The isolation of ribosome-associated transcripts is a powerful approach for deep profiling of cell type–specific transcripts, and particularly well-suited for quantitative analysis of transcript isoforms. This method employs conditional ribosome epitope-tagging in genetic作者: 捐助 時間: 2025-3-28 00:53
,FACS-Based Neuronal Cell Type–Specific RNA Isolation and Alternative Splicing Analysis,ributes to the marvelous complexity of the transcriptome in neurons. Given the differential expression of alternative splicing regulators and diversity in alternative splicing programs in neuronal subpopulations, it is urgent and necessary to develop methods to efficiently isolate diverse subgroups 作者: adj憂郁的 時間: 2025-3-28 06:07
,Quantitative Measurement of Alternatively Spliced RNA Isoform?Levels,ps) that include versus exclude a particular alternative RNA segment. The ratio measurement to study alternative splicing regulation can be confounded when alternative isoforms undergo differential RNA decay, for example, nonsense-mediated mRNA decay (NMD). Isoform-centric quantification is more inf作者: 執(zhí)拗 時間: 2025-3-28 09:05
Analysis of Splicing Regulation by Third-Generation Sequencing,cent transcripts. The regulation of exon inclusion by alternative splicing is one of the main sources of this diversity, which leads to the expansion of the proteome. The portfolio of alternative transcripts remains largely underestimated. Improvement of the sequencing technologies has enhanced the 作者: 連系 時間: 2025-3-28 14:16 作者: 否認(rèn) 時間: 2025-3-28 18:29 作者: 時代錯誤 時間: 2025-3-28 20:57 作者: 軍火 時間: 2025-3-29 00:04 作者: Cultivate 時間: 2025-3-29 04:44 作者: Vulnerable 時間: 2025-3-29 07:49 作者: 滴注 時間: 2025-3-29 11:52 作者: 雇傭兵 時間: 2025-3-29 15:43
Quantitative Detection of Protein Splice Variants by Selected Reaction Monitoring (SRM) Mass Spectr tools to specifically detect and quantify proteoforms (Smith et al., Nat Methods 10:186–187, 2013) is a major impediment to functional studies. Recently, biological mass spectrometry (MS) has undergone impressive advances (Mann, Nat Rev Mol Cell Biol 17:678, 2016), including the generation of a hig作者: 可憎 時間: 2025-3-29 21:11 作者: engrossed 時間: 2025-3-30 02:29
Genome-Wide CRISPR Screening to Identify Mammalian Factors that Regulate Intron Retention,a wide variety of genes controlled by IR, few techniques are available to identify regulators of IR in an unbiased manner. Here, we describe a CRISPR knockout screening method that can be applied to uncover regulators of IR. This method uses GFP reporter constructs containing a retained intron from 作者: 走調(diào) 時間: 2025-3-30 04:18
Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins, To elucidate the effect of a specific RBP on bound RNA, it can be artificially recruited to a specific site on a reporter mRNA that can be followed by a variety of methods. In this so-called tethering assay, the protein of interest (POI) is fused to the coat protein of the MS2 bacteriophage and exp作者: foodstuff 時間: 2025-3-30 09:58
,Probing Liquid–Liquid Phase Separation of RNA-Binding Proteins In Vitro and In Vivo,cellular content and compartmentalize biochemical reactions, in particular many processes involving RNA. This protocol is aimed at readers new to the LLPS field who want to test their protein or cellular structure of interest. We describe the basic principles of liquid–liquid phase separation, and o作者: 懶惰人民 時間: 2025-3-30 15:16
Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals, or bone marrow cells is possible by chemical ligation PCR. This method works independently of chemical modifications of the oligonucleotide and/or its conjugations to lipid or peptide moieties. Moreover, metabolization intermediates can be detected by mass spectrometry. Together with a readout assa作者: Entrancing 時間: 2025-3-30 17:45
Peter Scheiffele,Oriane MaugerIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts作者: 婚姻生活 時間: 2025-3-30 23:41 作者: –LOUS 時間: 2025-3-31 02:06
https://doi.org/10.1007/978-1-0716-2521-7Gene regulation; Proteome diversification; Transcript dynamics; Therapeutic strategies; High-resolution 作者: seduce 時間: 2025-3-31 07:44 作者: 去才蔑視 時間: 2025-3-31 12:11
In Situ Imaging of mRNA Splicing Variants by SpliceRCA,ngle-molecule resolution, discriminating splicing isoforms with single-base precision as well as analyzing the subcellular localization of transcripts. With this technology, it is possible to study cell heterogeneity of gene expression, potentially helping to decipher rich diversity in posttranscriptional function and assist clinical monitoring.作者: disrupt 時間: 2025-3-31 14:08
https://doi.org/10.1007/978-3-319-10536-9 of the genome but also enables complex mechanisms for the post-transcriptional control of gene expression. Regulation of alternative splicing entails a combinatorial interplay between an abundance of .-acting splicing factors, .-acting regulatory sequence elements and their concerted effects on the作者: 巧辦法 時間: 2025-3-31 18:15
Finance and Capital Markets Serieseutics that can modulate splicing has been dominated by antisense oligonucleotides (ASOs) and small molecule compounds, with each platform achieving remarkably effective results in the clinic. The success of RNA-targeting drugs has led to the exploration of new strategies to expand the repertoire of作者: municipality 時間: 2025-3-31 23:28 作者: CONE 時間: 2025-4-1 02:06 作者: 改正 時間: 2025-4-1 09:26
The Dictionary of Drugs: Chemical Dataps) that include versus exclude a particular alternative RNA segment. The ratio measurement to study alternative splicing regulation can be confounded when alternative isoforms undergo differential RNA decay, for example, nonsense-mediated mRNA decay (NMD). Isoform-centric quantification is more inf作者: Ascribe 時間: 2025-4-1 11:00 作者: 平躺 時間: 2025-4-1 15:28
R. G. Mirimanov,N. E. Sirotina,V. I. Neymanell/tissue types and disease, and what roles different AS events play. To facilitate AS research, we have created the computational . framework, which comprises a series of complementary software and resources that we describe in this chapter. The . framework is especially designed to aid biomedical作者: Explicate 時間: 2025-4-1 21:36 作者: 不妥協(xié) 時間: 2025-4-2 01:39
https://doi.org/10.1007/978-3-642-98090-9 transcriptome-wide sequencing technologies highlight the remarkable extent of this regulation in metazoans and allow for RNA isoforms to be profiled in increasingly small biological samples and with a growing confidence. Understanding biological functions of sample-specific transcripts is a major c作者: 不感興趣 時間: 2025-4-2 03:45 作者: 否認(rèn) 時間: 2025-4-2 08:07 作者: Cytology 時間: 2025-4-2 13:08
Part III Food Definitions and Formulationsngle-molecule resolution, discriminating splicing isoforms with single-base precision as well as analyzing the subcellular localization of transcripts. With this technology, it is possible to study cell heterogeneity of gene expression, potentially helping to decipher rich diversity in posttranscrip作者: 有權(quán)威 時間: 2025-4-2 17:34
https://doi.org/10.1007/978-1-4419-9713-5and the mechanisms controlling alternative splicing in specific cellular contexts. Reporters that recapitulate alternative splicing patterns of endogenous transcripts have served as excellent tools for dissecting regulatory mechanisms of splicing. In this chapter, we describe a two-color fluorescent作者: 圓柱 時間: 2025-4-2 21:46
