標題: Titlebook: Agrobacterium Protocols; Volume II Kan Wang Book 2007Latest edition Humana Press 2007 DNA.Flora.algae.arabidopsis thaliana.fungi.genetic en [打印本頁] 作者: 警察在苦笑 時間: 2025-3-21 17:04
書目名稱Agrobacterium Protocols影響因子(影響力)
書目名稱Agrobacterium Protocols影響因子(影響力)學科排名
書目名稱Agrobacterium Protocols網(wǎng)絡公開度
書目名稱Agrobacterium Protocols網(wǎng)絡公開度學科排名
書目名稱Agrobacterium Protocols被引頻次
書目名稱Agrobacterium Protocols被引頻次學科排名
書目名稱Agrobacterium Protocols年度引用
書目名稱Agrobacterium Protocols年度引用學科排名
書目名稱Agrobacterium Protocols讀者反饋
書目名稱Agrobacterium Protocols讀者反饋學科排名
作者: Limerick 時間: 2025-3-21 20:29
Cassava (, Crantz)ted transformation systems are routinely used to engineer cassava. These systems use either somatic embryos or friable embryogenic calli. This paper presents detailed protocols for the transformation of cassava using primary somatic embryos. The effects of explant types, tissue culture conditions, a作者: PAN 時間: 2025-3-22 03:49 作者: 六邊形 時間: 2025-3-22 06:36 作者: 不合 時間: 2025-3-22 11:55
Bermudagrass (, spp.)ent protocol that allows for the generation of a large number of transgenic bermudagrass plants, bypassing the callus formation phase. Stolon nodes are infected and co-cultivated with . harboring pCAMBIA binary vectors. Hygromycin phosphotransferase gene (.) is used as the selectable marker and hygr作者: 艱苦地移動 時間: 2025-3-22 14:30
Perennial Ryegrass (, L.)ein (sgfpS65T) reporter gene is introduced in combination with the .II selectable marker gene into axillary bud derived embryogenic calli of perennial ryegrass (. L.) by co-cultivation with . strain AGL0 harboring binary vector pYF132. Following the co-cultivation calli are cultured for 48 h in liqu作者: HARP 時間: 2025-3-22 17:47
Switchgrass (, L.)So far, switchgrass is the only warm season grass that has been transformed with .. We have developed a highly efficient system for transformation of different switchgrass explants utilizing the . strain AGL1 carrying the binary vector pDM805, containing the phosphinotricin acetyltransferase (.) and作者: 竊喜 時間: 2025-3-22 23:31
Tall Fescue (, Schreb.) chapter describes a protocol that allows for the generation of large number of transgenic tall fescue plants by .-mediated transformation. Embryogenic calli induced from caryopsis are used as explants for inoculation with .. The . strain used is EHA105. Hygromycin phosphotransferase gene (.) is use作者: 過濾 時間: 2025-3-23 02:50 作者: Campaign 時間: 2025-3-23 06:03 作者: 小卒 時間: 2025-3-23 11:40 作者: 打火石 時間: 2025-3-23 14:35 作者: 怎樣才咆哮 時間: 2025-3-23 21:48
Pine (,)fers. The advantages of our method are the year round availability of seed and that over 80% of genotypes can be easily regenerated from the mature cotyledon explants. The transformation efficiency (i.e., the number of transformed shoots regenerated from excised cotyledons) is 1.7% and, as with othe作者: 裝飾 時間: 2025-3-24 00:28
Poplar (, spp.)ecalcitrant. Many protocols that previously have been reported were developed for a specific genotype or species. Thus, it has often been necessary to re-optimize a protocol each time research is initiated with a new genotype. The method presented in this chapter has been effective for a wide variet作者: Mystic 時間: 2025-3-24 02:38 作者: 易受刺激 時間: 2025-3-24 08:47
1064-3745 sing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for作者: cortisol 時間: 2025-3-24 11:50
,Z, Zai’s Theory of Vital Energy,resents detailed protocols for the transformation of cassava using primary somatic embryos. The effects of explant types, tissue culture conditions, and bacterial and plasmid related factors on transformation efficiency are discussed.作者: 失敗主義者 時間: 2025-3-24 16:59 作者: 指令 時間: 2025-3-24 19:52 作者: 高原 時間: 2025-3-25 00:03
Cassava (, Crantz)resents detailed protocols for the transformation of cassava using primary somatic embryos. The effects of explant types, tissue culture conditions, and bacterial and plasmid related factors on transformation efficiency are discussed.作者: 擋泥板 時間: 2025-3-25 04:22
American Elm (,) American elm. Leaf pieces containing the midvein and petiole are used for explants. . strain EHA105 is used for transformation, with the binary vector pSE39, containing CaMV35S/.II as a selectable marker, ACS2/ESF39A as a putative resistance enhancing gene, and CaMV35S/GUS as a reporter.作者: 具體 時間: 2025-3-25 10:29
Pine (,)tyledon explants. The transformation efficiency (i.e., the number of transformed shoots regenerated from excised cotyledons) is 1.7% and, as with other . transformation methods, the majority of transgene integrations are single copy. Critical factors for success are survival of the cotyledons, . strain, and selection pressure after cocultivation.作者: Lumbar-Spine 時間: 2025-3-25 13:09
Dao Companions to Chinese Philosophy explants), the efficiency of transformation can be considerably improved in cv. Beniazuma. The high efficiency in the transformation of stem explants suggests that the transformation protocol described in this chapter warrants testing for routine stable transformation of diverse varieties of sweet potato.作者: 使害羞 時間: 2025-3-25 16:20 作者: 極小量 時間: 2025-3-25 23:33
Z, Xi and the Five Masters of Northern Songd as the selectable marker and hygromycin is used as the selection agent. Calli resistant to hygromycin are obtained after 4–6 wk of selection. Soil-grown tall fescue plants can be regenerated 4–5 mo after .-mediated transformation.作者: JUST 時間: 2025-3-26 02:51 作者: Cerumen 時間: 2025-3-26 05:40
Eucalyptus (.) genes into the plant nuclear genome are primarily verified by histochemical analysis and polymerase chain reaction (PCR). Modifications of this protocol to use in mature tissues derived from elite trees and other . species are also described.作者: cushion 時間: 2025-3-26 09:52
Book 2007Latest editionany other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric作者: 攤位 時間: 2025-3-26 14:48
Sweet Potato [, (L.) Lam.] explants), the efficiency of transformation can be considerably improved in cv. Beniazuma. The high efficiency in the transformation of stem explants suggests that the transformation protocol described in this chapter warrants testing for routine stable transformation of diverse varieties of sweet potato.作者: Contracture 時間: 2025-3-26 17:00
Bermudagrass (, spp.)omycin is used as the selection agent. Green shoots are directly produced from infected stolon nodes 4 to 5 wk after hygromycin selection. Without callus formation and with minimum tissue culture, this procedure allowed us to obtain well-rooted transgenic plantlets in only 7 wk and greenhouse-grown plants in only 9 wk.作者: FLIT 時間: 2025-3-26 20:59
Tall Fescue (, Schreb.)d as the selectable marker and hygromycin is used as the selection agent. Calli resistant to hygromycin are obtained after 4–6 wk of selection. Soil-grown tall fescue plants can be regenerated 4–5 mo after .-mediated transformation.作者: 楓樹 時間: 2025-3-27 01:35
Agrobacterium Protocols978-1-59745-131-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 希望 時間: 2025-3-27 07:10 作者: Proponent 時間: 2025-3-27 11:34
Tae Hyun Kim,Mark Csikszentmihalyiecalcitrant. Many protocols that previously have been reported were developed for a specific genotype or species. Thus, it has often been necessary to re-optimize a protocol each time research is initiated with a new genotype. The method presented in this chapter has been effective for a wide variety of poplar genotypes.作者: Fortuitous 時間: 2025-3-27 16:22 作者: 支形吊燈 時間: 2025-3-27 19:14 作者: Halfhearted 時間: 2025-3-28 01:15 作者: convulsion 時間: 2025-3-28 03:32 作者: Arthropathy 時間: 2025-3-28 07:36
,Z, Zai’s Theory of Vital Energy,ted transformation systems are routinely used to engineer cassava. These systems use either somatic embryos or friable embryogenic calli. This paper presents detailed protocols for the transformation of cassava using primary somatic embryos. The effects of explant types, tissue culture conditions, a作者: Mawkish 時間: 2025-3-28 12:10 作者: 沙草紙 時間: 2025-3-28 15:55 作者: pus840 時間: 2025-3-28 19:09
R, Ji’s “On Comprehending the ,”ent protocol that allows for the generation of a large number of transgenic bermudagrass plants, bypassing the callus formation phase. Stolon nodes are infected and co-cultivated with . harboring pCAMBIA binary vectors. Hygromycin phosphotransferase gene (.) is used as the selectable marker and hygr作者: 審問 時間: 2025-3-29 01:59
Language and Nothingness in W, Biein (sgfpS65T) reporter gene is introduced in combination with the .II selectable marker gene into axillary bud derived embryogenic calli of perennial ryegrass (. L.) by co-cultivation with . strain AGL0 harboring binary vector pYF132. Following the co-cultivation calli are cultured for 48 h in liqu作者: 分開 時間: 2025-3-29 06:44
Dao Companion to ZHU Xi’s PhilosophySo far, switchgrass is the only warm season grass that has been transformed with .. We have developed a highly efficient system for transformation of different switchgrass explants utilizing the . strain AGL1 carrying the binary vector pDM805, containing the phosphinotricin acetyltransferase (.) and作者: Mobile 時間: 2025-3-29 09:40 作者: tattle 時間: 2025-3-29 14:31
Dao Companion to ZHU Xi’s Philosophyneral protocol for the establishment of embryogenic cell cultures, .-mediated transformation, selection, and regeneration of transgenic turfgrass plants. Embryogenic callus is initiated from mature seeds, maintained by visual selection, and infected with an . strain (LBA4404) that contains either an作者: phase-2-enzyme 時間: 2025-3-29 16:14
Dao Companions to Chinese Philosophyr modifying American elm or enhancing disease resistance are significant. This protocol describes transformation and tissue culture techniques used on American elm. Leaf pieces containing the midvein and petiole are used for explants. . strain EHA105 is used for transformation, with the binary vecto作者: dominant 時間: 2025-3-29 20:32
Roger T. Ames,Henry Rosemont Jr.are inoculated with AGL1 strain harbouring the plasmid pBINUbiGUSint, which carries the .II and . genes. Evidence of stable transgene integration is obtained by polymerase chain reaction for .II and . genes, Southern blotting and expression of the . gene. The transgenic embryos are germinated and su作者: 貴族 時間: 2025-3-30 01:44
Dao Companions to Chinese Philosophyplants. Hypocotyl segments or cotyledon pieces from in vitro seedlings are used as starting materials. The explants are inoculated and cocultivated with a disarmed, binary strain of . CIB542 harboring a mini Ti plasmid, pBI121. A modified Gamborg’s B5 medium is used as the basal culture medium throu作者: 北極熊 時間: 2025-3-30 06:53
Cultivating the Self in Concert with Othersfers. The advantages of our method are the year round availability of seed and that over 80% of genotypes can be easily regenerated from the mature cotyledon explants. The transformation efficiency (i.e., the number of transformed shoots regenerated from excised cotyledons) is 1.7% and, as with othe作者: Fraudulent 時間: 2025-3-30 12:16 作者: 開始從未 時間: 2025-3-30 14:33 作者: magnanimity 時間: 2025-3-30 16:53
Kan WangIncludes supplementary material: 作者: Derogate 時間: 2025-3-31 00:25
Methods in Molecular Biologyhttp://image.papertrans.cn/a/image/151690.jpg作者: 織布機 時間: 2025-3-31 04:28 作者: Hearten 時間: 2025-3-31 07:30
Potato (, L.)method, and excised independent shoots rooting from the cut end of the stem after two further subcultures on kanamycin are 95% certain to be transformed. The transgenic status can be confirmed by molecular analysis and the plants grown on for tuber production enabling a wide spectrum of further stud作者: cumber 時間: 2025-3-31 09:35
