標(biāo)題: Titlebook: Agrobacterium Protocols; Volume 2 Kan Wang Book 2015Latest edition Springer Science+Business Media New York 2015 Agrobacterium.Agrobacteriu [打印本頁(yè)] 作者: corrode 時(shí)間: 2025-3-21 17:56
書(shū)目名稱(chēng)Agrobacterium Protocols影響因子(影響力)
作者: 強(qiáng)化 時(shí)間: 2025-3-21 21:50 作者: brassy 時(shí)間: 2025-3-22 01:43
Edmund S. K. Fung,Kenneth Kai-chung Yungter some of the major pests have been enthusiastically and widely accepted by the farmers in three of the major producing countries, i.e., China, India, and the USA. Considering the constraints related to its production and the wide variety of products derived from the cotton plant, it offers severa作者: 燒烤 時(shí)間: 2025-3-22 05:29
Dao Companions to Chinese Philosophy and unstable seed yield in semiarid lands unsuitable for the food production and infestation to diseases. Genetic engineering is promising to improve various agronomic traits in . and to understand the molecular functions of key . genes for molecular breeding. We describe a protocol routinely follo作者: photopsia 時(shí)間: 2025-3-22 11:31 作者: 可商量 時(shí)間: 2025-3-22 14:29 作者: 誰(shuí)在削木頭 時(shí)間: 2025-3-22 18:47
https://doi.org/10.1007/978-90-481-2927-0pathogens by heterologous expression in transgenic carrot tissues. The protocols for carrot transformation with . NPR1 (.) are described in this chapter, using the herbicide resistance gene ., which encodes phosphinothricin acetyltransferase, as a selectable marker. In this protocol, petiole segment作者: 傻 時(shí)間: 2025-3-23 00:40
Dao Companion to Daoist Philosophyz) transformation have facilitated the effective generation of stably transformed cassava plants with favorable traits. .-mediated transformation of friable, embryogenic callus has evolved to become the most widely used approach and has been adopted by research laboratories in Africa. This procedure作者: RAGE 時(shí)間: 2025-3-23 05:11 作者: 盤(pán)旋 時(shí)間: 2025-3-23 06:15
Nobukuni Koyasu 子安宣邦,Barry D. Stebenf taro is more efficient than the particle bombardment transformation method based on current research. The development of a regeneration system starting from taro shoot tip explants could produce dasheen mosaic virus (DsMV)-free plantlets. Highly regenerative calluses could be developed from DsMV-f作者: 致命 時(shí)間: 2025-3-23 13:40 作者: cumber 時(shí)間: 2025-3-23 14:58 作者: Climate 時(shí)間: 2025-3-23 19:53 作者: 遺傳 時(shí)間: 2025-3-23 23:12 作者: novelty 時(shí)間: 2025-3-24 04:43 作者: Ablation 時(shí)間: 2025-3-24 08:53
Dao Companion to Korean Confucian Philosophyfor wine but also for fresh fruit, dried fruit, and juice production. Wine is by far the major product of grapes, and the focus of this chapter is on wine grape cultivars. Grapevine cultivars of . L. have a reputation for producing premium quality wines. These premium quality wines are produced from作者: 反抗者 時(shí)間: 2025-3-24 11:41
Philippe Major,Milan Matthiesenmediated transformation in the melon (.) were from the early 1990s (Fang and Grumet, Plant Cell Rep, 9: 160–164, 1990; Dong et al., Nat Biotechnol 9: 858–863, 1991; Valles and Lasa, Plant Cell Rep 13: 145–148, 1994). These early studies described three problems that decreased the efficiency of trans作者: 不能仁慈 時(shí)間: 2025-3-24 16:22
Potato (, L.)he . family. This chapter describes protocols for the genetic transformation of three species of potato: . subsp. . (Desiréé), . subsp. . (Blue potato), and . subsp. . using internodal segments as explants.作者: 致詞 時(shí)間: 2025-3-24 19:25
Edmund S. K. Fung,Kenneth Kai-chung YungWithin this chapter we outline an .-mediated transformation method for . using 4-day-old cotyledonary explants and the genotype R-o-18. Transformation efficiencies are typically achieved in the region of 1 % (based on 2 PCR-positive independent shoots from 200 inoculated explants). This system has been developed to work with gentamicin selection.作者: exhibit 時(shí)間: 2025-3-24 23:44 作者: 解決 時(shí)間: 2025-3-25 06:10
Kan WangIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts作者: Multiple 時(shí)間: 2025-3-25 09:27
Methods in Molecular Biologyhttp://image.papertrans.cn/a/image/151689.jpg作者: esthetician 時(shí)間: 2025-3-25 12:00
Agrobacterium Protocols978-1-4939-1658-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: Nonporous 時(shí)間: 2025-3-25 16:13 作者: 暴行 時(shí)間: 2025-3-25 21:09
Philippe Major,Milan Matthiesenformation: tetraploidy, chimeras, and escape. Using a liquid culture system for somatic embryogenesis, Akasaka-Kenedy et al. (Plant Sci 166: 763–769, 2004) overcame these problems and established an efficient transformation system; the protocol introduced in this chapter is based on this method.作者: Confess 時(shí)間: 2025-3-26 01:54
1064-3745 ation advice from the experts.Rapid changes and significant progress have been made in the .Agrobacterium. field, such as genetically transforming plants for both basic research purposes and agricultural development. In .Agrobacterium Protocols, Third Edition, Volumes 1 .and. 2., a team of leading e作者: GORGE 時(shí)間: 2025-3-26 07:37
Dao Companion to Korean Confucian Philosophy cocultivation medium; selection and regeneration of transformed shoots are carried out on selection medium containing 50 mg/L kanamycin (Km) and 250 mg/L timentin (or cefotaxime) for 3–5 months. In this protocol, the optimal media for shoot proliferation and shoot regeneration from leaf explants are genotype dependent.作者: opinionated 時(shí)間: 2025-3-26 11:22
Cherry cocultivation medium; selection and regeneration of transformed shoots are carried out on selection medium containing 50 mg/L kanamycin (Km) and 250 mg/L timentin (or cefotaxime) for 3–5 months. In this protocol, the optimal media for shoot proliferation and shoot regeneration from leaf explants are genotype dependent.作者: 拍下盜公款 時(shí)間: 2025-3-26 16:18 作者: Neuropeptides 時(shí)間: 2025-3-26 16:57
Melon (,)formation: tetraploidy, chimeras, and escape. Using a liquid culture system for somatic embryogenesis, Akasaka-Kenedy et al. (Plant Sci 166: 763–769, 2004) overcame these problems and established an efficient transformation system; the protocol introduced in this chapter is based on this method.作者: 變形詞 時(shí)間: 2025-3-27 00:02
Dao Companions to Chinese Philosophy with . strain EHA105 harboring pBI121 plant binary vector, which contains . as plant selectable marker and . as reporter. The putative transformed plants are selected on kanamycin, and stable integration of transgene(s) is confirmed by histochemical GUS assay, polymerase chain reaction, and Southern hybridization.作者: growth-factor 時(shí)間: 2025-3-27 04:27 作者: OGLE 時(shí)間: 2025-3-27 06:43 作者: 跟隨 時(shí)間: 2025-3-27 10:58 作者: COM 時(shí)間: 2025-3-27 15:32 作者: 的染料 時(shí)間: 2025-3-27 19:38 作者: Constituent 時(shí)間: 2025-3-27 23:00
Cassava (, Crantz) binary expression cassette is used to transform this tissue, which is regenerated via cotyledons and shoot organogenesis to produce rooted in vitro plantlets. This chapter details each step of the procedure using the model cultivar 60444 and provides supplementary notes to successfully produce transgenic cassava.作者: 粗俗人 時(shí)間: 2025-3-28 06:07 作者: ARCHE 時(shí)間: 2025-3-28 07:46 作者: blackout 時(shí)間: 2025-3-28 12:58 作者: 轎車(chē) 時(shí)間: 2025-3-28 16:00
Dao Companion to Korean Confucian Philosophyd to shoot multiplication medium where they elongated in shoots and proliferated. Elongated transgenic shoots were rooted in a medium containing 70 μM kanamycin. Rooted plants were acclimatized following standard procedures. This constitutes the only transformation protocol described for apricot clonal tissues and one of the few of ..作者: 愚笨 時(shí)間: 2025-3-28 22:45 作者: 憤怒歷史 時(shí)間: 2025-3-28 22:55 作者: Ischemia 時(shí)間: 2025-3-29 03:03 作者: Hormones 時(shí)間: 2025-3-29 10:19
Apricot (, L.)d to shoot multiplication medium where they elongated in shoots and proliferated. Elongated transgenic shoots were rooted in a medium containing 70 μM kanamycin. Rooted plants were acclimatized following standard procedures. This constitutes the only transformation protocol described for apricot clonal tissues and one of the few of ..作者: 放氣 時(shí)間: 2025-3-29 12:46 作者: cutlery 時(shí)間: 2025-3-29 17:50
1064-3745 and other important plant species. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists 978-1-4939-4544-3978-1-4939-1658-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 角斗士 時(shí)間: 2025-3-29 20:56 作者: 突襲 時(shí)間: 2025-3-30 00:58 作者: habile 時(shí)間: 2025-3-30 05:48 作者: 膝蓋 時(shí)間: 2025-3-30 10:13 作者: Graduated 時(shí)間: 2025-3-30 13:47
Dao Companion to Korean Confucian Philosophyo the original genome would leave the traditional characteristics of the cultivar intact. However, a reliable transformation system is required for a successful transgenic approach to grapevine improvement. There are three criteria for achieving an efficient .-mediated transformation system: (1) the作者: 果核 時(shí)間: 2025-3-30 17:10 作者: arbiter 時(shí)間: 2025-3-30 21:23 作者: SLUMP 時(shí)間: 2025-3-31 04:14
Chestnut, American (, (Marsh.) Borkh.)able embryogenic transformation events per gram of somatic embryo tissue, a considerable improvement over the 20 transformation events per gram we reported in 2006 (Maynard et al. American chestnut (. (Marsh.) Borkh.) . protocols, 2nd ed., 2006). We have regenerated nearly 100 of these events, conta作者: 完整 時(shí)間: 2025-3-31 06:16
Grapevine (, L,)o the original genome would leave the traditional characteristics of the cultivar intact. However, a reliable transformation system is required for a successful transgenic approach to grapevine improvement. There are three criteria for achieving an efficient .-mediated transformation system: (1) the作者: 厭惡 時(shí)間: 2025-3-31 11:50 作者: BATE 時(shí)間: 2025-3-31 14:30
