標(biāo)題: Titlebook: Agrobacterium Protocols; Volume 1 Kan Wang Book 2015Latest edition Springer Science+Business Media New York 2015 Agrobacterium-mediated tra [打印本頁] 作者: 領(lǐng)口 時間: 2025-3-21 18:51
書目名稱Agrobacterium Protocols影響因子(影響力)
作者: 發(fā)起 時間: 2025-3-21 21:27
The small grass . has attributes that make itd containing the desired transformation sequence. Following cocultivation, callus is transferred to selective media to identify and amplify the transgenic tissue. After 2–5 weeks on selection media, transgenic callus is moved onto regeneration media for 2–4 weeks until plantlets emerge. Plantlets ar作者: travail 時間: 2025-3-22 02:50 作者: Trabeculoplasty 時間: 2025-3-22 06:48
Maize, Tropical (, L.) methods used with model genotypes adapted to temperate areas are not necessarily efficient with tropical lines. . transformation is the method of choice since it has been first achieved in 1996. In this report, we describe a transformation method used successfully with several tropical maize lines.作者: Synthesize 時間: 2025-3-22 09:35 作者: Definitive 時間: 2025-3-22 13:38
The Shelleys and Dante’s Matildaignaling compound acetosyringone at comparatively high concentration and the use of cysteine to reduce the extent of cellular oxidation upon agroinfection. In addition, the use of liquid, rather than solid, cocultivation medium promotes the throughput of the method. The protocol has delivered well o作者: Acetabulum 時間: 2025-3-22 19:42 作者: BILL 時間: 2025-3-22 22:05
1064-3745 legume plants, vegetable plants, and three model plant species: .Brachypodium distachyon, Medicago truncatula., and .Setaria viridis.. It also updates a chapter for vector construction, a step critical to a suc978-1-4939-4541-2978-1-4939-1695-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 動物 時間: 2025-3-23 01:46
Book 2015Latest editionurposes and agricultural development. In .Agrobacterium Protocols, Third Edition, Volumes 1 .and. 2., a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops a作者: Aspirin 時間: 2025-3-23 07:56 作者: 煤渣 時間: 2025-3-23 12:55 作者: 事與愿違 時間: 2025-3-23 15:27 作者: fiscal 時間: 2025-3-23 18:30
The Philosophy of Confucius’ Disciples between 40 and 90 % of the immature embryos, thus infected in our tests. All regenerants were normal in morphology and fully fertile. About half of the transformed plants carried single copy of the transgene, which are inherited by the progeny in a Mendelian fashion.作者: diathermy 時間: 2025-3-24 00:31 作者: Offensive 時間: 2025-3-24 03:28
Transformation Using Cotyledonary Explants rapid production of transgenic plants from Jemalong A17 and other genotypes. Transgenic plants can be regenerated and established in the greenhouse in only 3–4 months after .-mediated transformation. Transformation frequency was in the range of 5–12 %.作者: Palter 時間: 2025-3-24 09:44
Transformation Using Leaf Explantson protocols rely on the use of highly regenerable lines obtained by recurrent in vitro culture selection. These protocols are based on . transformation of . followed by somatic embryogenesis-mediated plant regeneration. We describe here the protocol developed for . R108-1 (c3).作者: 精美食品 時間: 2025-3-24 11:58 作者: 合同 時間: 2025-3-24 15:19
Wheat (, L.) Transformation Using Mature Embryosof mature embryos as a target tissue has the advantage of only requiring good-quality seed as the starting material. Here we describe a transformation method based on the .-mediated transformation of callus cultures derived from mature wheat embryos of the genotype Bobwhite S56.作者: 恃強(qiáng)凌弱的人 時間: 2025-3-24 20:48 作者: 畸形 時間: 2025-3-24 23:49 作者: 我邪惡 時間: 2025-3-25 06:22
Introduction: Chinese Philosophy of Logic,sing in the protocol one of the newly developed pBract or pCAMBIA (.) vector systems which carry different selection cassettes. The commonly used selection genes .II, ., and . were proved to be applicable for oat transformation and might be used as needed.作者: Dysarthria 時間: 2025-3-25 09:46 作者: 許可 時間: 2025-3-25 11:44 作者: Enliven 時間: 2025-3-25 16:20 作者: 充滿人 時間: 2025-3-25 23:30
Finger millet [, (L.) Gaertn.]ing cocultivation helps for the better recovery of transgenic plants. This protocol is very useful for the efficient production of transgenic plants in finger millet through .-mediated transformation.作者: CHARM 時間: 2025-3-26 02:10 作者: 善辯 時間: 2025-3-26 05:17 作者: INCUR 時間: 2025-3-26 08:43
Rice, Japonica (, L.)nbare with an emphasis on tissue desiccation. The explants consist of callus derived from mature seeds which are cocultivated on filter paper postinfection. Hygromycin selection is used for the recovery of subsequent genetically engineered events.作者: 遭受 時間: 2025-3-26 15:17 作者: 正面 時間: 2025-3-26 18:21 作者: 做事過頭 時間: 2025-3-27 00:43
Interest in plant model systems for genetic, methods for .-mediated transformation of seed-derived callus. Vectors used for gene constructs contained the hygromycin phosphotransferase gene as a selectable marker. The transition of transgenic lines to soil was straightforward; plants started to flower in 1–3 weeks, with seeds ready to harvest approximately 5 weeks later.作者: 帶傷害 時間: 2025-3-27 02:24 作者: STANT 時間: 2025-3-27 08:26 作者: 正常 時間: 2025-3-27 09:55 作者: Ingenuity 時間: 2025-3-27 14:32 作者: 閃光你我 時間: 2025-3-27 19:02 作者: 鄙視 時間: 2025-3-27 22:37
Transformation Using Cotyledonary Explants is difficult to regenerate from callus cultures. By using cotyledons as explants for . infection and direct shoot formation, this protocol allows for rapid production of transgenic plants from Jemalong A17 and other genotypes. Transgenic plants can be regenerated and established in the greenhouse i作者: reflection 時間: 2025-3-28 04:13 作者: 正常 時間: 2025-3-28 09:42
Interest in plant model systems for genetic, n time, small stature, and simple growth requirements. A model species would be especially advantageous for the studies of C. photosynthetic grasses, which currently present practical challenges. These include long seed-to-seed generation times, and because of their large size at maturity they requi作者: 斜谷 時間: 2025-3-28 11:20 作者: APO 時間: 2025-3-28 15:08 作者: instate 時間: 2025-3-28 19:49
Maize (, L.)ormation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an . strain carrying a standard binary vector. In addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plan作者: antipsychotic 時間: 2025-3-28 23:16
Maize, Tropical (, L.)n staple of the diet; 95 % of the maize grown is consumed directly as human food and as an important source of income for the resource-poor rural population. The biotechnological approach to engineer biotic and abiotic traits implies the availability of an efficient plant transformation method. The 作者: construct 時間: 2025-3-29 03:12
Finger millet [, (L.) Gaertn.]we describe an optimized protocol for the .-mediated transformation of finger millet variety GPU 45. . strain LBA4404 harboring plasmid pCAMBIA1301 which contains hygromycin phosphotransferase (.) as selectable marker gene and β-glucuronidase (GUS) as reporter gene has been used. This protocol utili作者: 使迷醉 時間: 2025-3-29 08:50 作者: 施舍 時間: 2025-3-29 15:23 作者: consent 時間: 2025-3-29 17:46 作者: 身心疲憊 時間: 2025-3-29 22:51 作者: Infraction 時間: 2025-3-30 02:27
Wheat (, L.) Transformation Using Immature Embryosgrown in a well-conditioned greenhouse were pretreated with centrifuging and cocultivated with .. Transgenic wheat plants were obtained routinely from between 40 and 90 % of the immature embryos, thus infected in our tests. All regenerants were normal in morphology and fully fertile. About half of t作者: forthy 時間: 2025-3-30 04:40 作者: REIGN 時間: 2025-3-30 11:35
Maize (, L.)ormation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an . strain carrying a standard binary vector. In addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plants in the greenhouse.作者: TOXIN 時間: 2025-3-30 15:23 作者: 糾纏 時間: 2025-3-30 20:13 作者: CHASE 時間: 2025-3-30 23:21
