標題: Titlebook: Agrobacterium Protocols; Volume I Kan Wang Book 2006Latest edition Humana Press 2006 Agrobacterium tumefaciens.Arabidopsis thaliana.DNA.Flo [打印本頁] 作者: ambulance 時間: 2025-3-21 18:43
書目名稱Agrobacterium Protocols影響因子(影響力)
書目名稱Agrobacterium Protocols影響因子(影響力)學科排名
書目名稱Agrobacterium Protocols網(wǎng)絡公開度
書目名稱Agrobacterium Protocols網(wǎng)絡公開度學科排名
書目名稱Agrobacterium Protocols被引頻次
書目名稱Agrobacterium Protocols被引頻次學科排名
書目名稱Agrobacterium Protocols年度引用
書目名稱Agrobacterium Protocols年度引用學科排名
書目名稱Agrobacterium Protocols讀者反饋
書目名稱Agrobacterium Protocols讀者反饋學科排名
作者: 嫌惡 時間: 2025-3-21 20:22 作者: Ringworm 時間: 2025-3-22 04:02
Three Methods for the Introduction of Foreign DNA into ,ormable plant cells through the use of T-DNA binary vectors. Three methods are commonly used. Transformation with purified plasmid can be done with either electroporation or a simple freeze/thaw transformation method. Alternatively, a mobilizable plasmid can be placed into . using the triparental ma作者: 杠桿支點 時間: 2025-3-22 05:15 作者: 認識 時間: 2025-3-22 11:59
Nucleic Acid Extraction from , Strainsells often requires the manipulation and analysis of nucleic acids present in recombinant . strains. Here we present dependable methods for the isolation of genomic (total) DNA, mega-plasmid DNA, shuttle or binary plasmid DNA, and RNA. In addition, we provide a simple method for the electronic trans作者: 教唆 時間: 2025-3-22 14:59
Virulence Gene Inductionion as a two-component regulatory system to sense particular phenolic compounds synthesized by wounded plant tissues. Induction by these phenolic compounds, in the presence of certain neutral or acid sugars, results in activation of other . genes, leading to the processing of T-DNA from the Ti-plasm作者: 謙卑 時間: 2025-3-22 19:31
Floral Dip Transformation Methodsequently set seed, and transgenic plants are then selected among the progeny seedlings. Because no plant tissue culture is required, somaclonal variation is avoided, and the procedure can be performed easily by nonspecialists. Success rates are high: it is common that 1% of the progeny seedlings ar作者: 情節(jié)劇 時間: 2025-3-23 00:16 作者: 滑稽 時間: 2025-3-23 02:08
Transformation Using Cotyledon Explantse genotype Jemalong A17 is in progress. By using cotyledons as explants for . infection and direct shoot formation, this protocol allows for rapid production of transgenic plants from A17 and other genotypes. Transgenic plants can be regenerated and established in the greenhouse in only 3-4 mo after作者: 善于騙人 時間: 2025-3-23 09:17
Transformation Using Root Explantstions, including nodulation and mycorrhizal colonization. The development of different transformation methods is an important aspect for functional genomic studies in the species. This protocol describes an efficient system for generating transgenic plants from . roots based on .-mediated transforma作者: left-ventricle 時間: 2025-3-23 13:45
(,)for rapid evaluation of transgenes in higher plants. This protocol has a number of desirable attributes: readily available explant material, high efficiency, and a relatively quick turnaround time. The in vitro regeneration scheme of the leaf disks is prolific and follows an indirect organogenic dif作者: Inferior 時間: 2025-3-23 17:32 作者: 粘 時間: 2025-3-23 18:12
Barley (, L.)would be of great value. Barley has been transformed using microprojectile bombardment and by direct gene transfer to protoplasts, but neither method has been able to produce fertile transformants in large numbers with simple transgene integration characteristics. .-mediated transformation was first作者: 錢財 時間: 2025-3-24 01:43
Maize (, L.)ormation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an . strain carrying a standard binary vector. In addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plan作者: Callus 時間: 2025-3-24 03:25 作者: micronutrients 時間: 2025-3-24 08:21 作者: 主動 時間: 2025-3-24 12:31 作者: 在前面 時間: 2025-3-24 16:31 作者: 恩惠 時間: 2025-3-24 19:07 作者: fender 時間: 2025-3-25 01:27
https://doi.org/10.1007/978-3-031-44093-9ther electroporation or a simple freeze/thaw transformation method. Alternatively, a mobilizable plasmid can be placed into . using the triparental mating method. Here we present three detailed protocols for . strain construction using electroporation, the freeze/thaw method of transformation, and triparental mating.作者: BOOM 時間: 2025-3-25 03:20 作者: morale 時間: 2025-3-25 09:16
Conclusion: A Sea of Differencesmediated transformation of somatic tissue. Such investigations require a reproducible, quantitative assay system to determine transformation frequency. We describe here an . root transformation protocol that can be used to determine transformation frequencies for wild-type and mutant . strains and for various . wild-type and mutant lines.作者: synovium 時間: 2025-3-25 11:41
Introduction: A Mediterranean Comedy,duction of transgenic plants from A17 and other genotypes. Transgenic plants can be regenerated and established in the greenhouse in only 3-4 mo after .-mediated transformation. Transformation frequency is in the range of 5-12%.作者: 袖章 時間: 2025-3-25 17:55 作者: 輕浮女 時間: 2025-3-25 22:43
Culture and Maintenance of , Strains of careless technique than because of strain difficulties. Here we describe a few of the complex and defined media that have been successfully used in the growth of agrobacteria including some that are semiselective for agrobacteria. Finally, we present methods suitable for short- and long-term storage of . strains.作者: 發(fā)炎 時間: 2025-3-26 02:56 作者: 清晰 時間: 2025-3-26 07:34
Nucleic Acid Extraction from , Strainsion of genomic (total) DNA, mega-plasmid DNA, shuttle or binary plasmid DNA, and RNA. In addition, we provide a simple method for the electronic transfer of shuttle plasmids from . to . for use when their low copy number in . impedes plasmid isolation from that strain.作者: 節(jié)省 時間: 2025-3-26 10:41
Transformation of , Rootsmediated transformation of somatic tissue. Such investigations require a reproducible, quantitative assay system to determine transformation frequency. We describe here an . root transformation protocol that can be used to determine transformation frequencies for wild-type and mutant . strains and for various . wild-type and mutant lines.作者: 拉開這車床 時間: 2025-3-26 13:05
Transformation Using Cotyledon Explantsduction of transgenic plants from A17 and other genotypes. Transgenic plants can be regenerated and established in the greenhouse in only 3-4 mo after .-mediated transformation. Transformation frequency is in the range of 5-12%.作者: 債務 時間: 2025-3-26 20:43 作者: HAWK 時間: 2025-3-27 00:50 作者: 配偶 時間: 2025-3-27 03:32
https://doi.org/10.1007/978-3-031-44093-9r describes in detail a method to integrate a gene of interest into the ./. locus of the . C58 chromosome. The integrated gene is stably maintained as single copy per cell without the need for selection.作者: faculty 時間: 2025-3-27 08:41 作者: 衰老 時間: 2025-3-27 12:28
https://doi.org/10.1057/9780230508491ts per year. Moreover, seed size and quantity per event permit monitoring of segregation in Petri plates, and sufficient biomass can be accrued from an individual plant, which can be either clonally propagated or allowed to self-pollinate or easily outcrossed.作者: 宿醉 時間: 2025-3-27 17:16 作者: 廢止 時間: 2025-3-27 20:37 作者: 世俗 時間: 2025-3-28 00:06 作者: Flatter 時間: 2025-3-28 05:40
(,)ts per year. Moreover, seed size and quantity per event permit monitoring of segregation in Petri plates, and sufficient biomass can be accrued from an individual plant, which can be either clonally propagated or allowed to self-pollinate or easily outcrossed.作者: PON 時間: 2025-3-28 07:23
Book 2006Latest editionany other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric作者: 或者發(fā)神韻 時間: 2025-3-28 12:29 作者: humectant 時間: 2025-3-28 17:46
https://doi.org/10.1007/978-3-030-40771-1ormation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an . strain carrying a standard binary vector. In addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plants in the greenhouse.作者: Proclaim 時間: 2025-3-28 19:12
https://doi.org/10.1007/978-3-476-05061-8 of other chemoorganotrophs will usually work for agrobacteria as well. Problems with culture or strain maintenance will occur more frequently because of careless technique than because of strain difficulties. Here we describe a few of the complex and defined media that have been successfully used i作者: BYRE 時間: 2025-3-28 23:31 作者: 專心 時間: 2025-3-29 06:02 作者: climax 時間: 2025-3-29 08:53 作者: reperfusion 時間: 2025-3-29 12:07 作者: 上下倒置 時間: 2025-3-29 15:44
Dante and Italy in British Romanticismion as a two-component regulatory system to sense particular phenolic compounds synthesized by wounded plant tissues. Induction by these phenolic compounds, in the presence of certain neutral or acid sugars, results in activation of other . genes, leading to the processing of T-DNA from the Ti-plasm作者: chemical-peel 時間: 2025-3-29 23:10
Introduction: A Mediterranean Comedy,sequently set seed, and transgenic plants are then selected among the progeny seedlings. Because no plant tissue culture is required, somaclonal variation is avoided, and the procedure can be performed easily by nonspecialists. Success rates are high: it is common that 1% of the progeny seedlings ar作者: 我們的面粉 時間: 2025-3-30 03:48
Conclusion: A Sea of Differencesed transformation using “flower dip” and “vacuum infiltration” protocols. However, . has also become a major system to investigate the mechanism of .-mediated transformation of somatic tissue. Such investigations require a reproducible, quantitative assay system to determine transformation frequency作者: ETCH 時間: 2025-3-30 05:52
Introduction: A Mediterranean Comedy,e genotype Jemalong A17 is in progress. By using cotyledons as explants for . infection and direct shoot formation, this protocol allows for rapid production of transgenic plants from A17 and other genotypes. Transgenic plants can be regenerated and established in the greenhouse in only 3-4 mo after作者: 擴音器 時間: 2025-3-30 09:04
Conclusion: A Sea of Differencestions, including nodulation and mycorrhizal colonization. The development of different transformation methods is an important aspect for functional genomic studies in the species. This protocol describes an efficient system for generating transgenic plants from . roots based on .-mediated transforma作者: RAGE 時間: 2025-3-30 14:11
https://doi.org/10.1057/9780230508491for rapid evaluation of transgenes in higher plants. This protocol has a number of desirable attributes: readily available explant material, high efficiency, and a relatively quick turnaround time. The in vitro regeneration scheme of the leaf disks is prolific and follows an indirect organogenic dif作者: 無目標 時間: 2025-3-30 18:21
https://doi.org/10.1057/9780230508491sgenics. To this end we have developed both tissue culture and non-tissue culture-based methodologies for the production of transgenic roots on wild-type shoots (composite plants). Composite plants are generated by inoculating wild-type shoots with ., which subsequently induces the formation of tran作者: Forehead-Lift 時間: 2025-3-30 20:47 作者: 有惡意 時間: 2025-3-31 01:10 作者: Migratory 時間: 2025-3-31 07:39
https://doi.org/10.1007/978-3-030-40771-1wing demand of the ever-increasing population, more sustained production of indica-type rice is needed. In addition, because of the high per capita consumption of indica rice, improvement of any traits including its nutritive value may have a significant positive health outcome for the rice-consumin作者: Parabola 時間: 2025-3-31 10:44
