標(biāo)題: Titlebook: Aging; Methods and Protocol Sean P. Curran Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Drosophila.Yeast.li [打印本頁(yè)] 作者: mobility 時(shí)間: 2025-3-21 17:27
書(shū)目名稱Aging影響因子(影響力)
書(shū)目名稱Aging影響因子(影響力)學(xué)科排名
書(shū)目名稱Aging網(wǎng)絡(luò)公開(kāi)度
書(shū)目名稱Aging網(wǎng)絡(luò)公開(kāi)度學(xué)科排名
書(shū)目名稱Aging被引頻次
書(shū)目名稱Aging被引頻次學(xué)科排名
書(shū)目名稱Aging年度引用
書(shū)目名稱Aging年度引用學(xué)科排名
書(shū)目名稱Aging讀者反饋
書(shū)目名稱Aging讀者反饋學(xué)科排名
作者: right-atrium 時(shí)間: 2025-3-21 21:52
Analysis of Lifespan in ,: Low- and High-Throughput Approaches,population of animals over time and score viability based on movement. We previously developed an alternative approach, called the Replica Set method, to quantitatively measure lifespan of . in a high-throughput manner. The replica set method allows a single investigator to screen more treatments or作者: 故意釣到白楊 時(shí)間: 2025-3-22 03:47
,A?Simple Apparatus for Individual , Culture,es. This system, the “worm corral,” is compatible with high-resolution brightfield and fluorescent microscopy, allowing imaging of fluorescent transgenes and morphological phenotypes from hatch until death. These culture devices can be constructed on the benchtop with commercially available reagents作者: HAWK 時(shí)間: 2025-3-22 06:51 作者: Introduction 時(shí)間: 2025-3-22 10:23 作者: 腐敗 時(shí)間: 2025-3-22 15:40 作者: Eructation 時(shí)間: 2025-3-22 18:41 作者: 陪審團(tuán) 時(shí)間: 2025-3-22 23:38 作者: Feigned 時(shí)間: 2025-3-23 05:17 作者: irreducible 時(shí)間: 2025-3-23 07:03
,Quantitative Profiling of Lipid Species in , with Gas Chromatography–Mass Spectrometry,orate findings from common staining methodologies, providing great resolution on the lipid species altered in abundance in aging, genetic mutants, or with dietary or pharmacologic manipulation. Here we describe a method to quantitate relative abundance of fatty acids in total worm lipid extracts, as作者: 填滿 時(shí)間: 2025-3-23 12:30
Assessing Insulin and Glucose Tolerance in the Aging Mouse,ls. Mice, the most commonly used species to study the biological processes that regulate aging and its associated functional decline, have helped researchers to identify pathways, mechanisms, and genes that regulate aging-related diseases and aging itself and thus are intervention targets. Changes i作者: grounded 時(shí)間: 2025-3-23 17:40 作者: acolyte 時(shí)間: 2025-3-23 18:45
Measurements of Innate Immune Function in ,ation, and protective host responses. Experimentally, . can be infected with a wide variety of human pathogens, as well as with natural pathogens of worms that were isolated from wild-caught nematodes. Here, we focus on an experimental model of bacterial pathogenesis that utilizes the human opportun作者: Fester 時(shí)間: 2025-3-23 23:06 作者: MOCK 時(shí)間: 2025-3-24 05:47
Transcriptional Profiling of , Adult Cells and Tissues with Age,g those that arise with age. Since . is a premier model system used to study the molecular and cellular regulators of adult and aging phenotypes, cell type- and tissue-specific approaches are needed to characterize the genome-wide expression changes associated with these responses. Here we describe 作者: Feigned 時(shí)間: 2025-3-24 09:33
Assessing Tissue-Specific Autophagy Flux in Adult ,nd has been linked to longevity in many species, including the nematode .. Study of the physiological roles of autophagy during . aging requires methods for the spatiotemporal analysis of autophagy. Here we describe a method for assessing autophagic flux in multiple tissues of . by quantifying the p作者: 郊外 時(shí)間: 2025-3-24 13:46 作者: GRIN 時(shí)間: 2025-3-24 17:52
,Staining and Quantification of β-Amyloid Pathology in Transgenic Mouse Models of Alzheimer’s Diseasn of β-amyloid protein (Aβ), which is widely hypothesized to have a key role in AD pathogenesis. Quantification of Aβ in transgenic mice typically is accomplished through both biochemical and histochemical approaches. In this chapter, we describe two techniques for the histological detection of Aβ, 作者: generic 時(shí)間: 2025-3-24 19:23 作者: 思想 時(shí)間: 2025-3-25 01:48 作者: BIDE 時(shí)間: 2025-3-25 07:00 作者: judiciousness 時(shí)間: 2025-3-25 07:47
John G. Borkowski,Fredi P. Büchel well as by drugs and dietary components, to reveal basic and conserved mechanisms of aging. Here methods are presented for Drosophila lifespan assay, including media preparation, supplementation of media with various drugs, culturing of the flies, passaging flies and recording deaths, and the analysis of lifespan data.作者: agitate 時(shí)間: 2025-3-25 12:50 作者: 小故事 時(shí)間: 2025-3-25 18:15
https://doi.org/10.1007/978-981-19-5197-8, and by extension, age-related biological pathways. Here, we present a method for measuring the lifespan of . in 96-well microtiter plates to identify and study pharmacologically active molecules that extend lifespan. The format of this assay is readily adapted for automated liquid handling systems and imaging of phenotypes.作者: 不規(guī)則 時(shí)間: 2025-3-25 22:40
Harmonic Currents and Canonical Complexes,, producing a defined number of sperm during larval development before switching exclusively to oogenesis. Here we show a method of assaying both oocyte and sperm quality from a single population of animals.作者: 類似思想 時(shí)間: 2025-3-26 04:03 作者: Sinus-Node 時(shí)間: 2025-3-26 06:26
Juliana de Melo Bezerra,Celso Massaki Hirataaccomplished through both biochemical and histochemical approaches. In this chapter, we describe two techniques for the histological detection of Aβ, immunostaining with Aβ antibodies and staining with the amyloid dye thioflavin S, and its quantification using digital imaging.作者: famine 時(shí)間: 2025-3-26 10:05
Measuring the Replicative Lifespan of , Using the HYAA Microfluidic Platform, microfluidic systems have been developed to automate the dissection process, significantly accelerating RLS determination. Here, we describe a detailed protocol of RLS measurement using a ommercially available microfluidic system based on the HYAA chip design, which enables data collection of up to 8000 cells in a single experiment.作者: TOM 時(shí)間: 2025-3-26 14:47
,A?Simple Apparatus for Individual , Culture,nes and morphological phenotypes from hatch until death. These culture devices can be constructed on the benchtop with commercially available reagents and standard laboratory equipment, making this an attainable solution for most labs.作者: FLUSH 時(shí)間: 2025-3-26 20:27
Analysis of , Lifespan, well as by drugs and dietary components, to reveal basic and conserved mechanisms of aging. Here methods are presented for Drosophila lifespan assay, including media preparation, supplementation of media with various drugs, culturing of the flies, passaging flies and recording deaths, and the analysis of lifespan data.作者: 想象 時(shí)間: 2025-3-26 21:45 作者: 贊成你 時(shí)間: 2025-3-27 01:46 作者: acrobat 時(shí)間: 2025-3-27 07:21
Methods for Assessing Fertility in , from a Single Population,, producing a defined number of sperm during larval development before switching exclusively to oogenesis. Here we show a method of assaying both oocyte and sperm quality from a single population of animals.作者: CHASM 時(shí)間: 2025-3-27 11:43 作者: maintenance 時(shí)間: 2025-3-27 13:41
,Staining and Quantification of β-Amyloid Pathology in Transgenic Mouse Models of Alzheimer’s Diseasaccomplished through both biochemical and histochemical approaches. In this chapter, we describe two techniques for the histological detection of Aβ, immunostaining with Aβ antibodies and staining with the amyloid dye thioflavin S, and its quantification using digital imaging.作者: 想象 時(shí)間: 2025-3-27 20:09
Metabolic Assessment of Lipid Abundance and Distribution,e in lipid homeostasis due to its short lifespan and remarkably well-conserved metabolic pathways. Due to their ease of use and similarities, there have been numerous developments in methodologies to study intracellular lipid abundance and tissue distribution in ..作者: 緊張過(guò)度 時(shí)間: 2025-3-27 23:23
,Quantitative Profiling of Lipid Species in , with Gas Chromatography–Mass Spectrometry, well as a method that quantitates fatty acids following separation into neutral lipid pools (triacylglycerols and cholesteryl esters) versus more polar lipids (phospholipids) by solid-phase extraction (SPE).作者: Corral 時(shí)間: 2025-3-28 02:21 作者: 發(fā)酵 時(shí)間: 2025-3-28 09:15 作者: OCTO 時(shí)間: 2025-3-28 10:31
Book 2020eir respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...?Authoritative and cutting-edge,? .Aging: Methods and Protocols .aims to ensure successful results in the further study of this vital field..作者: 偶然 時(shí)間: 2025-3-28 18:17
Divisors and Harmonic Currents,e in lipid homeostasis due to its short lifespan and remarkably well-conserved metabolic pathways. Due to their ease of use and similarities, there have been numerous developments in methodologies to study intracellular lipid abundance and tissue distribution in ..作者: Inflammation 時(shí)間: 2025-3-28 22:47 作者: gerontocracy 時(shí)間: 2025-3-29 01:24 作者: 善于 時(shí)間: 2025-3-29 04:03
Surface Applications for Security Analysisool of autophagic vesicles using fluorescently labelled?Atg8/LGG-1 reporters upon autophagy inhibition using bafilomycin A. (BafA). This methodology has revealed that autophagic activity varies in different cell types of . during aging.作者: exigent 時(shí)間: 2025-3-29 08:52
Book 2020 optimize health. Chapters guide readers through different animal models and methodologies to examine aging at the organism, tissue, cellular, and molecular levels across the lifespan. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to th作者: 制定 時(shí)間: 2025-3-29 14:00
Mnemonic-Device Instruction with Adultsvation point, rather than a single sample over time as with “traditional” longitudinal methods. The protocols provided here describe both the traditional experimental approach and the Replica Set method, as well as practical considerations for each.作者: arthroplasty 時(shí)間: 2025-3-29 16:54 作者: Servile 時(shí)間: 2025-3-29 23:01
Analysis of Lifespan in ,: Low- and High-Throughput Approaches,vation point, rather than a single sample over time as with “traditional” longitudinal methods. The protocols provided here describe both the traditional experimental approach and the Replica Set method, as well as practical considerations for each.作者: 燈泡 時(shí)間: 2025-3-30 02:42 作者: Eclampsia 時(shí)間: 2025-3-30 05:41 作者: AXIS 時(shí)間: 2025-3-30 10:23 作者: 叫喊 時(shí)間: 2025-3-30 16:05 作者: 淘氣 時(shí)間: 2025-3-30 19:25
Tamsin Meaney,Tony Trinick,Uenuku FairhallChromatin Immunoprecipitation is a regularly used method to detect DNA–protein interaction in diverse biological samples. Here we describe the application of ChIP for histone modifications in adult-stage . somatic cells.作者: Friction 時(shí)間: 2025-3-30 21:37
Chromatin Immunoprecipitation from , Somatic Cells,Chromatin Immunoprecipitation is a regularly used method to detect DNA–protein interaction in diverse biological samples. Here we describe the application of ChIP for histone modifications in adult-stage . somatic cells.作者: 泥土謙卑 時(shí)間: 2025-3-31 04:13 作者: Hiatus 時(shí)間: 2025-3-31 08:00 作者: Nmda-Receptor 時(shí)間: 2025-3-31 12:28
https://doi.org/10.1007/978-1-4612-5522-2e replicative lifespan (RLS) is measured by manually dissecting mother cells from daughter cells, which is a very tedious process. Since 2012, several microfluidic systems have been developed to automate the dissection process, significantly accelerating RLS determination. Here, we describe a detail作者: 粗野 時(shí)間: 2025-3-31 13:37
Mnemonic-Device Instruction with Adultspopulation of animals over time and score viability based on movement. We previously developed an alternative approach, called the Replica Set method, to quantitatively measure lifespan of . in a high-throughput manner. The replica set method allows a single investigator to screen more treatments or
