標題: Titlebook: Advanced Fluorescence Microscopy; Methods and Protocol Peter J. Verveer Book 2015 Springer Science+Business Media New York 2015 biological [打印本頁] 作者: 日月等 時間: 2025-3-21 17:20
書目名稱Advanced Fluorescence Microscopy影響因子(影響力)
書目名稱Advanced Fluorescence Microscopy影響因子(影響力)學科排名
書目名稱Advanced Fluorescence Microscopy網絡公開度
書目名稱Advanced Fluorescence Microscopy網絡公開度學科排名
書目名稱Advanced Fluorescence Microscopy被引頻次
書目名稱Advanced Fluorescence Microscopy被引頻次學科排名
書目名稱Advanced Fluorescence Microscopy年度引用
書目名稱Advanced Fluorescence Microscopy年度引用學科排名
書目名稱Advanced Fluorescence Microscopy讀者反饋
書目名稱Advanced Fluorescence Microscopy讀者反饋學科排名
作者: Exaggerate 時間: 2025-3-21 23:55
Die ertragsteuerliche Organschaftquestions. We explain technical aspects of sample preparation and image acquisition that will help in obtaining good diffraction-unlimited pictures. We also present embedding techniques adapted for ultrathin sectioning, which allow optimal 3D resolutions in virtually all biological preparations.作者: Antigen 時間: 2025-3-22 01:57
https://doi.org/10.1007/978-3-642-51985-7commercial system. We explain how TIRF can be combined with other microscopy modalities and describe how to use TIRF to study processes such as endocytosis, exocytosis, and focal adhesion dynamics. Finally, we provide a step-by-step guide to imaging and analyzing focal adhesion dynamics in a migrating cell using TIRF microscopy.作者: 軌道 時間: 2025-3-22 06:19 作者: PTCA635 時間: 2025-3-22 09:02 作者: 性別 時間: 2025-3-22 16:08 作者: Antagonist 時間: 2025-3-22 18:38
https://doi.org/10.1007/978-3-322-85310-3signaling events inside living cells, e.g., Protein–Protein interactions. In this chapter, we describe the calibration of both time-correlated single-photon counting (TCSPC) and frequency domain (FD) FLIM systems and the acquisition and analysis of FLIM-FRET data for investigating Protein–Protein interactions in living cells.作者: Intersect 時間: 2025-3-22 23:37
https://doi.org/10.1007/978-3-322-85310-3ipt language. The simulation tool fits very well in a systems biology research setting that aims to maintain an interactive cycle of experiment-driven modelling and model-driven experimentation: the model and the experiment are in the same simulation. The full program can be obtained by request to the authors.作者: 冷峻 時間: 2025-3-23 02:42 作者: 短程旅游 時間: 2025-3-23 09:10 作者: jaunty 時間: 2025-3-23 11:18 作者: 顧客 時間: 2025-3-23 17:06 作者: Amenable 時間: 2025-3-23 22:02
Die ertragsteuerliche Organschaftluorescent proteins (PA-FPs) as PALM probes. After reviewing the basics of 2D PALM, we detail astigmatic and multiphoton imaging approaches well suited to working with PA-FPs. We also discuss the use of open-source localization software appropriate for 3D PALM.作者: Constrain 時間: 2025-3-24 00:46 作者: 滔滔不絕地說 時間: 2025-3-24 03:40
https://doi.org/10.1007/978-3-662-26592-5ration, owing to the longer excitation wavelength required and to the ability to collect scattered emission photons as a useful signal. It also minimizes photodamage because lower energy photons are used and because fluorescence is confined to the geometrical focus of the laser spot. 2PE is therefor作者: orthopedist 時間: 2025-3-24 09:44
Kinder in einer globalisierten Welte protocol allows the recording of all phases of spheroid formation in three dimensions, including cell proliferation, aggregation, and compaction. We employ the human hepatic cell line HepaRG transfected with the fusion protein H2B-GFP, i.e., a fluorescing histone. The protocol allows monitoring th作者: venous-leak 時間: 2025-3-24 14:42
Ulrich Hagemann,Harald Seehausenomated preparation of “ready to transfect” multiwell plates and cell arrays, on which cells can be grown which are then reversely transfected with one type of siRNA in every individual well or spot. Additionally, different microscope types for screening approaches are compared and considerations abo作者: 專橫 時間: 2025-3-24 15:01 作者: Endometrium 時間: 2025-3-24 19:25
https://doi.org/10.1007/978-3-322-85310-3s to advanced clinical diagnosis. Fluorescence lifetime imaging microscopy (FLIM) is now routinely used in the biological sciences to monitor dynamic signaling events inside living cells, e.g., Protein–Protein interactions. In this chapter, we describe the calibration of both time-correlated single-作者: Heterodoxy 時間: 2025-3-25 00:50
https://doi.org/10.1007/978-3-322-85310-3antitative analysis of FRAP assays to study these. The program is straightforward in its implementation and can be instructed through an intuitive script language. The simulation tool fits very well in a systems biology research setting that aims to maintain an interactive cycle of experiment-driven作者: 思想 時間: 2025-3-25 03:49
Die ertragsteuerliche Organschaft a brief introduction to the technique, this chapter presents a protocol including background information in order to measure and quantify the molecular interaction of two signaling proteins inside the living cell using fluorescence cross-correlation spectroscopy.作者: 純樸 時間: 2025-3-25 09:01 作者: 離開真充足 時間: 2025-3-25 14:00 作者: Overdose 時間: 2025-3-25 15:56
Die ertragsteuerliche Organschaftively interfere in a common focus. This yields a diffraction pattern in the direction of the optical axis, which essentially consists of a main focal spot accompanied by two smaller side lobes. At optimal conditions, the main peak of this so-called point spread function has a full width at half maxi作者: contrast-medium 時間: 2025-3-25 21:35
Die ertragsteuerliche Organschaftn far-field fluorescence microscopy. The first nanoscopy method successfully applied to conventional biological probes was stimulated emission depletion microscopy (STED). It is based on a physical principle that instantly delivers diffraction-unlimited images, with no need for further computational作者: Reverie 時間: 2025-3-26 04:03
Die ertragsteuerliche Organschaft ~50–100 nm axial resolution. Although such techniques were originally demonstrated on single imaging planes close to the coverslip surface, recent technical developments now enable the 3D imaging of whole fixed cells. We describe methods for converting a 2D PALM into a system capable of acquiring s作者: WAX 時間: 2025-3-26 08:08 作者: LIMN 時間: 2025-3-26 08:53
Das Schreibwerk beim Zugdienstefamily. A blue light-sensing LOV (light, oxygen, or voltage) domain derived from . (oat) phototropin was fused to the N-terminus of a constitutively active mutant of Rac, via an α-helix (Jα) that is conserved among plant phototropins. The fused LOV domain occluded binding of downstream effectors to 作者: overwrought 時間: 2025-3-26 14:41 作者: effrontery 時間: 2025-3-26 17:18 作者: 宴會 時間: 2025-3-26 21:59
Ulrich Hagemann,Harald Seehausenomated preparation of “ready to transfect” multiwell plates and cell arrays, on which cells can be grown which are then reversely transfected with one type of siRNA in every individual well or spot. Additionally, different microscope types for screening approaches are compared and considerations about the information workflow are made.作者: FOLLY 時間: 2025-3-27 04:11
Die ertragsteuerliche Organschaft a brief introduction to the technique, this chapter presents a protocol including background information in order to measure and quantify the molecular interaction of two signaling proteins inside the living cell using fluorescence cross-correlation spectroscopy.作者: Engulf 時間: 2025-3-27 07:39 作者: consent 時間: 2025-3-27 10:43
Peter J. VerveerIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia作者: Climate 時間: 2025-3-27 17:31 作者: 睨視 時間: 2025-3-27 20:08 作者: 全部逛商店 時間: 2025-3-28 01:08 作者: 射手座 時間: 2025-3-28 04:55
Front Matterie impliziert eine enge Zusammenarbeit mit einer Vielzahl unterschiedlichster Stellen der Organisation und ein breites Spektrum an Qualifikationen. So ist der Portfoliomanager Experte für den Markt und die Produkte in seinem Portfolio, Ansprechpartner für Controlling, Projektabteilungen, Vertrieb, v作者: 道學氣 時間: 2025-3-28 09:23
The Physical Basis of Total Internal Reflection Fluorescence (TIRF) Microscopy and Its Cellular Appd Futures zur Krisenbew?ltigung erfolgreich einsetzbar sind. Dafür habe ich für die zw?lf gr??ten Finanz- und Wirtschaftskrisen zwischen 1987 und 2022 Kurs-, Zins- und Volatilit?tsdaten analysiert und mit ihnen die Entwicklung von Portfolien mit und ohne derivaten Strategien rekonstruiert.?Mit der P作者: 法律 時間: 2025-3-28 11:23
Two-Photon Excitation Microscopy and Its Applications in Neuroscience,ve reduzieren l??t, so da? eine N?herungsl?sung durch Verwendung einer konstanten risikospezifischen Absicherungspr?mie m?glich wird. Diese vorteilhafte Situation wird jedoch bei einer realit?tsn?heren Betrachtung nicht gegeben sein. So k?nnen sowohl die einzelnen Fremdw?hrungskreditvolumina, als au作者: 吃掉 時間: 2025-3-28 15:55
Fluorescence Microscopy-Based RNA Interference Screening, Wagnisfinanzierungen nur einen kleinen Anteil am gesamten Kapitalmarkt ausmachen, haben sie gro?e volkswirtschaftliche Bedeutung über ihren Einfluss auf das Wirtschaftswachstum. Die Portfoliostrategie eines VCG, d.h. die Entscheidung darüber, in wie viele und in welche Wagnisse investiert werden so作者: 一再煩擾 時間: 2025-3-28 20:01 作者: IRK 時間: 2025-3-29 02:36
Analysis of Biomolecular Dynamics by FRAP and Computer Simulation,hrittenem kolorektalem Karzinom entwickeln Lebermetasen. Die arterielle Therapie der Leber hebt die relative Zytostatikakonzentration in den Tumorzellen gegenüber Normalzellen an, da Lebermetastasen überwiegend arteriell von der Leberarterie versorgt werden, w?hrend Hepatozyten über die V portae ver作者: chalice 時間: 2025-3-29 04:35
Fluorescence Correlation Spectroscopy,el fills the space between the cement grains, bridges between them, and thereby causes stiffening of the paste and its subsequent hardening. The continued formation of the gel gradually fills the capillary pores, the porosity of the paste decreases, and its strength is increased. However, the mechan作者: Ibd810 時間: 2025-3-29 08:42
Practical Structured Illumination Microscopy,the area of nuclear physics (CERN) in Copenhagen. He was a?fellow of CERN . the organization was officially created and at the same time a?lecturer in Lund..CERN was officially created on the 29 September 1954, after an intense period of preparations, involving many steps in several countries. The g作者: 寬容 時間: 2025-3-29 11:54
4Pi Microscopy of the Nuclear Pore Complex,m further afield. It provides education for what may be considered a better class of pupil than the mass schools which run according to the standard general curriculum, and consequently it and the host of other schools like it attract complaints that they are bastions of privilege. Some have argued 作者: APRON 時間: 2025-3-29 18:22
Application of STED Microscopy to Cell Biology Questions,hey have a more sacred connotation. They stand for the Short-Title Catalogue of English books printed before 1640. — that tool (a word beloved of researchers) so indispensable to those in the trade. A generation ago, in presenting the original edition, its editors, A. W. Pollard and G. R. Redgrave, 作者: debris 時間: 2025-3-29 22:56 作者: 觀察 時間: 2025-3-30 01:47
, Stochastic Optical Reconstruction Microscopy (,STORM),ies of participants to highlight the individual behind the dBased on an ethnographic study involving three families who live on a Midlands council housing estate, this book presents portraits of everyday lives - and the literacy practices that are part of them - as a way to explore the complex relat作者: Suppository 時間: 2025-3-30 07:12
