標(biāo)題: Titlebook: Adenovirus Methods and Protocols; Volume 1: Adenovirus William S. M. Wold,Ann E. Tollefson Book 2007Latest edition Humana Press 2007 DNA.EL [打印本頁] 作者: 粗略 時(shí)間: 2025-3-21 19:35
書目名稱Adenovirus Methods and Protocols影響因子(影響力)
書目名稱Adenovirus Methods and Protocols影響因子(影響力)學(xué)科排名
書目名稱Adenovirus Methods and Protocols網(wǎng)絡(luò)公開度
書目名稱Adenovirus Methods and Protocols網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Adenovirus Methods and Protocols被引頻次
書目名稱Adenovirus Methods and Protocols被引頻次學(xué)科排名
書目名稱Adenovirus Methods and Protocols年度引用
書目名稱Adenovirus Methods and Protocols年度引用學(xué)科排名
書目名稱Adenovirus Methods and Protocols讀者反饋
書目名稱Adenovirus Methods and Protocols讀者反饋學(xué)科排名
作者: COM 時(shí)間: 2025-3-21 20:37 作者: 為寵愛 時(shí)間: 2025-3-22 02:08
Kristina Reiss,Gerald Schmiederuction of viral DNA and viral DNA-protein complex, and transfection of mouse cells to obtain mouse adenovirus mutants are described in this chapter. Plaque purification, propagation, and titration of the mutant viruses are also presented.作者: 主動(dòng)脈 時(shí)間: 2025-3-22 07:48
,Einfüuhrung in die Systemtheorie,hods for generating subcutaneous tumors in cotton rats and assessing the efficacy of Ad vectors upon intratumoral injection. Also, we discuss procedures for determining the biodistribution of a replicating Ad in cotton rats.作者: BARK 時(shí)間: 2025-3-22 09:46 作者: 巨碩 時(shí)間: 2025-3-22 15:11
Fazit zu den wichtigsten Steuerarten,luding human cells, poorly. However, genetic modification of capsid proteins (e.g., fiber, pIX) has helped in increasing the utility of BAdV-3 as a vector for transducing nonbovine cells. Here, we will describe the methods used to construct recombinant BAdV-3 expressing chimeric fiber or chimeric pIX proteins.作者: BET 時(shí)間: 2025-3-22 21:01 作者: CRAFT 時(shí)間: 2025-3-22 22:11
Book 2007Latest edition, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad ve作者: Communal 時(shí)間: 2025-3-23 04:28
https://doi.org/10.1007/978-3-658-23827-8ffords one the opportunity to “detarget” adenovirus, to retarget adenovirus, and to alter immune recognition. In this chapter, we are presenting a terminal exon-replacement strategy that can be used to genetically manipulate capsid proteins expressed from the MLTU. An emphasis will be placed on manipulations of fiber as an intact terminal exon.作者: Tracheotomy 時(shí)間: 2025-3-23 07:50 作者: 蔑視 時(shí)間: 2025-3-23 09:43 作者: 委派 時(shí)間: 2025-3-23 15:01 作者: Altitude 時(shí)間: 2025-3-23 19:35
William S. M. Wold,Ann E. TollefsonDetailed description of quality control testing of vectors.Methods for determination of virus titers.Sensitive assays for testing viral DNA presence in samples.Developing new, permissive immunocompete作者: vasospasm 時(shí)間: 2025-3-23 22:54 作者: 想象 時(shí)間: 2025-3-24 02:35
Adenovirus Methods and Protocols978-1-59745-166-6Series ISSN 1543-1894 Series E-ISSN 1940-6037 作者: 亞麻制品 時(shí)間: 2025-3-24 09:59 作者: Merited 時(shí)間: 2025-3-24 11:45
https://doi.org/10.1007/978-3-8348-9122-8ll types used to propagate the more commonly used subgroup C serotypes (Ad2 and Ad5) and there is no standard plaque assay. Methods to propagate Ad40 in complementing cell lines and to evaluate infectivity and particle number are presented in this chapter.作者: 并排上下 時(shí)間: 2025-3-24 16:43
https://doi.org/10.57088/978-3-7329-8939-3 used to quantitate adenoviruses (such as the plaque assay or fluorescent focus assay) are time-consuming and subjective in their interpretation. Here we describe a flow cytometric method that eliminates these disadvantages and provides a quantitative and reliable method of focus-forming unit (FFU) assay.作者: aggravate 時(shí)間: 2025-3-24 20:10
Kristina Reiss,Gerald SchmiederDNA damage, and apoptosis. E4 is essential for viral growth in most cell lines. In this chapter, the current knowledge of the functions of six E4 products is summarized briefly. Protocols are presented for manipulation of E4, incorporation of E4 mutations into the viral genome, and growth of E4 muta作者: 動(dòng)脈 時(shí)間: 2025-3-25 01:51
Anwendungen der elementaren Zahlentheorie, deletion mutant. In general, the deletion mutant then must be separated physically from the helper for use in subsequent experiments. This chapter includes suggestions for selection of helper viruses, protocols for the production of stocks by complementation, and procedures for physical separation 作者: 預(yù)示 時(shí)間: 2025-3-25 06:44 作者: 容易做 時(shí)間: 2025-3-25 07:50 作者: BAIL 時(shí)間: 2025-3-25 14:16
Zahldarstellungen und Stellenwertsysteme,ailed method for the rapid construction of adenoviral vectors. The method described here uses homologous recombination machinery of . BJ5183 to construct plasmids used in generation of adenoviral vectors. With this method, no ligation steps are involved in generating the plasmids, and any region of 作者: HAIRY 時(shí)間: 2025-3-25 16:55 作者: critic 時(shí)間: 2025-3-25 21:02
Fazit zu den wichtigsten Steuerarten,enovirus (HAdV) and the promiscuous nature of the virus have made the use of nonhuman adenoviruses an attractive alternative. Moreover, readministration of viral vectors is often required to maintain therapeutic levels of transgene expression, resulting in vector-specific immune responses. Although 作者: 并入 時(shí)間: 2025-3-26 03:01 作者: BORE 時(shí)間: 2025-3-26 07:36 作者: 窗簾等 時(shí)間: 2025-3-26 10:04 作者: Harass 時(shí)間: 2025-3-26 13:22
https://doi.org/10.1007/978-3-8348-9122-8ll types used to propagate the more commonly used subgroup C serotypes (Ad2 and Ad5) and there is no standard plaque assay. Methods to propagate Ad40 in complementing cell lines and to evaluate infectivity and particle number are presented in this chapter.作者: 假裝是你 時(shí)間: 2025-3-26 17:00
,Einfüuhrung in die Systemtheorie,le. In this chapter we describe an immunocompetent, semi-permissive cotton rat tumor model to evaluate the safety and efficacy of oncolytic Ad vectors. With this model one can investigate the effect of the host immune system on the vector-tumor interaction as well as the vector’s effect on normal ho作者: 強(qiáng)壯 時(shí)間: 2025-3-26 21:46
https://doi.org/10.1007/978-3-8348-9122-8mitations. Because Ad replication is generally species-specific, oncolytic Ads are usually examined in immunodeficient mice bearing human xenograft tumors. However, this model suffers because the animals are immunodeficient and normal and cancerous mouse tissues are poorly permissive to human Ad rep作者: 蝕刻 時(shí)間: 2025-3-27 04:13 作者: Pudendal-Nerve 時(shí)間: 2025-3-27 07:21 作者: Overstate 時(shí)間: 2025-3-27 10:23 作者: recession 時(shí)間: 2025-3-27 13:38 作者: 調(diào)整 時(shí)間: 2025-3-27 21:17
Forum für Fachsprachen-ForschungA capture enzyme-linked immunosorbent assay (ELISA) was optimized for identification of mouse adenovirus type 1 in infected brain homogenates. The ELISA method allows for a much faster quantitation of virus in infected organs than plaque assays. Methods for organ homogenization and the subsequent capture ELISA are described in this chapter.作者: athlete’s-foot 時(shí)間: 2025-3-28 01:59 作者: 比喻好 時(shí)間: 2025-3-28 05:47 作者: 乳白光 時(shí)間: 2025-3-28 07:22
Evaluating Apoptosis in Tumor Cells Infected With Adenovirus Expressing p53 Tumor Suppressor Gene,978-3-662-07533-3作者: 倔強(qiáng)一點(diǎn) 時(shí)間: 2025-3-28 11:17 作者: 江湖郎中 時(shí)間: 2025-3-28 14:42 作者: flaunt 時(shí)間: 2025-3-28 21:27 作者: Cardioversion 時(shí)間: 2025-3-29 01:36 作者: voluble 時(shí)間: 2025-3-29 07:02
Construction of Capsid-Modified Recombinant Bovine Adenovirus Type 3, niveaux: communication très physique entre deux endroits, entre deux choses qui passent entre ces endroits, entre l’extérieur de l’homme et son intérieur, entre le niveau le plus élevé, le plus ?spirituel? de la grande communication de l’être avec les influences célestes.作者: Dissonance 時(shí)間: 2025-3-29 09:44 作者: Cpr951 時(shí)間: 2025-3-29 13:17
Growth and Purification of Enteric Adenovirus Type 40,mu’ohe = . [es] ., ndramu’ohe, oder ndra 1a’ohe = . [es] .. Ebenso kann das Perfectum ?no u’ohe? geradezu besagen ?ich habe [es] bereits gebracht?, und ein Perfectum kann auch ohne ?no? oder ?ho? gebildet werden (siehe die Formenlehre.) Weniger ist dies letztere der Fall bei den intransitiven Verben作者: 本土 時(shí)間: 2025-3-29 17:31
Immunocompetent, Semi-Permissive Cotton Rat Tumor Model for the Evaluation of Oncolytic Adenovirusear verfluchende und Rache für Sigurer gelobende Guerún des ?Brot?:.mag einen mit dem deutschen Sagenschlu? vertrauten Dichter verraten (weswegen man sein Gedicht neuerdings zu den jüngeren Liedern rechnet.), Kenntnis der Vorgeschichte setzt auch die Atlaqviea voraus, und es bleibt bestehen, da? die 作者: cogitate 時(shí)間: 2025-3-29 22:32
Use of the Syrian Hamster as an Animal Model for Oncolytic Adenovirus Vectors,Jahren beachtliche Fortschritte im Aufbau eines demokratischen, rechtsstaatlichen und marktwirtschaftlichen Gemeinwesens sowie eines effizienteren Staatsapparats machen konnte. Diese Reformen stellten eine radikale ordnungspolitische und institutionelle Neuausrichtung des Landes dar. Studien belegen作者: 宣誓書 時(shí)間: 2025-3-30 02:49
A Real-Time PCR Method to Rapidly Titer Adenovirus Stocks,oceeds on the basis of an assessment of the particular law of the sea dimension which the ICJ commented upon in its judgments. Six law of the sea issues are assessed commencing with the characterisation of maritime features, followed by the status of low-tide elevations, historic bays, internal wate作者: DEFT 時(shí)間: 2025-3-30 06:39
Detection and Quantitation of Subgroup C Adenovirus DNA in Human Tissue Samples by Real-Time PCR,y others. A theme running through is the thought of what is our favourite number..Written in an engaging and witty style and requiring only basic school mathematical knowledge, this book will appeal to both young and mature readers fascinated by the curiosities of numbers..作者: VAN 時(shí)間: 2025-3-30 08:54
Flow Cytometric Detection of Adenoviruses and Intracellular Adenovirus Proteins,terest in ‘middle-powerdom’ has emerged. The aim of this chapter is to consider the nature of the changes which have brought this about, what middle-power strategies are, and to discuss some problems to which they give rise. This will be done in the context of the Australian experience.作者: 老人病學(xué) 時(shí)間: 2025-3-30 16:11 作者: Decongestant 時(shí)間: 2025-3-30 20:29
Lineare und quadratische Kongruenzen,viral E1 and/or E4 transcription units can be specifically modified or deleted. Moreover, foreign genes can be introduced into the E1 and/or E4 regions, and expression of viral or therapeutic genes can be controlled by cell-type specific and/or inducible promoters.作者: 自戀 時(shí)間: 2025-3-30 23:19
Zahldarstellungen und Stellenwertsysteme,d in the targeted region, into BJ5183 cells. Homologous recombination in . generates plasmids containing the modified adenoviral genome. Recombinant virus is generated following release of the viral DNA sequences from the plasmid backbone and transfection into a producer cell line. With this method,作者: 上坡 時(shí)間: 2025-3-31 04:15 作者: Mumble 時(shí)間: 2025-3-31 07:16 作者: 出價(jià) 時(shí)間: 2025-3-31 09:32 作者: Hay-Fever 時(shí)間: 2025-3-31 13:23 作者: growth-factor 時(shí)間: 2025-3-31 20:05 作者: 裁決 時(shí)間: 2025-3-31 22:58 作者: extract 時(shí)間: 2025-4-1 05:41
Book 2007Latest editionmodifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-a
