標(biāo)題: Titlebook: Wnt Signaling; Methods and Protocol Quinn Barrett,Lawrence Lum Book 2016 Springer Science+Business Media New York 2016 Regenerative medicin [打印本頁] 作者: complicated 時(shí)間: 2025-3-21 16:25
書目名稱Wnt Signaling影響因子(影響力)
書目名稱Wnt Signaling影響因子(影響力)學(xué)科排名
書目名稱Wnt Signaling網(wǎng)絡(luò)公開度
書目名稱Wnt Signaling網(wǎng)絡(luò)公開度學(xué)科排名
書目名稱Wnt Signaling被引頻次
書目名稱Wnt Signaling被引頻次學(xué)科排名
書目名稱Wnt Signaling年度引用
書目名稱Wnt Signaling年度引用學(xué)科排名
書目名稱Wnt Signaling讀者反饋
書目名稱Wnt Signaling讀者反饋學(xué)科排名
作者: CHANT 時(shí)間: 2025-3-21 22:53
Jesung Moon,James F. Amatrudaation is by no means straightforward for in most cases the manner of allocation will be determined by expectations about future events, such as the life of a piece of plant or the success of a new development.作者: 執(zhí)拗 時(shí)間: 2025-3-22 01:06
Annastasia Simone Hyde,Brian I. Hang,Ethan Lee of a project. The most dominating stakeholder is the customer or the user of the software product being built by the Agile professionals, so a majority of the chapter is devoted to understanding how to engage them in project affairs from start to finish.作者: intelligible 時(shí)間: 2025-3-22 07:31 作者: EXCEL 時(shí)間: 2025-3-22 12:27
Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells,of inductive signals that are mediated by growth factors, including FGF, TGFβ, and Wnt. In this book chapter, we introduce a set of tools, methods, and protocols to specifically manipulate the Wnt signaling pathway with the intention of altering the cell fate outcome of hPSCs.作者: Esophagus 時(shí)間: 2025-3-22 15:32 作者: laceration 時(shí)間: 2025-3-22 20:59 作者: Gene408 時(shí)間: 2025-3-23 00:11 作者: BLAND 時(shí)間: 2025-3-23 05:00
Visualizing Wnt Palmitoylation in Single Cells,vides high sensitivity and subcellular resolution for detection of Wnt palmitoylation. It is also compatible with multiple imaging platforms, and is applicable to detecting palmitoylated forms of other fatty acylated proteins.作者: AMBI 時(shí)間: 2025-3-23 05:46 作者: 苦笑 時(shí)間: 2025-3-23 09:58 作者: Prostaglandins 時(shí)間: 2025-3-23 14:51
Use of Primary Calvarial Osteoblasts to Evaluate the Function of Wnt Signaling in Osteogenesis,e of the Wntless (Wls) gene (..). We previously used this approach to delete the Wntless gene by infecting with a Cre-expressing adenovirus, and to evaluate the effects of Wnt signaling loss on osteogenic potential in osteogenic medium with ascorbic acid. This detailed protocol is adaptable to use with any floxed allele.作者: 浮雕寶石 時(shí)間: 2025-3-23 20:37 作者: 埋葬 時(shí)間: 2025-3-24 01:56
,A Simple Method to Assess Abundance of the β-Catenin Signaling Pool in Cells,m of ICAT (.nhibitor of β-.tenin and .cf) as an affinity matrix. This method is more sensitive and quantitative than immunofluorescence and may be useful in studies that implicate TCF-independent signaling events.作者: indices 時(shí)間: 2025-3-24 02:56
Wnt-Dependent Control of Cell Polarity in Cultured Cells,ream signaling events that regulate these complex cellular processes are still poorly understood. The methods below describe assays to study Wnt5a-induced cell polarity in cultured cells, which may facilitate our understanding of these complex signaling pathways.作者: 嗎啡 時(shí)間: 2025-3-24 10:01 作者: ovation 時(shí)間: 2025-3-24 13:08
The Generation of Organoids for Studying Wnt Signaling,o study cellular signaling pathways, specifically Wnt signaling. Moreover, we provide a detailed protocol for lentiviral transduction and CRISPR/Cas9-mediated genome modification of organoid cultures.作者: curettage 時(shí)間: 2025-3-24 16:06
Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notor Wnt activities. Here, we provide detailed procedures for preparing enzymatic active Tiki and Notum proteins and the in vitro enzymatic reactions. We also describe a metabolic labeling and click chemistry method for detection of Wnt protein acylation.作者: 開玩笑 時(shí)間: 2025-3-24 20:11 作者: 可耕種 時(shí)間: 2025-3-24 23:41
Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In Vivo,s. This biochemical assay will facilitate chemically based studies focused on understanding the role of Tankyrase in cell fate reprogramming and tissue homeostasis and provide insights into the potential role of Wnt/β-catenin signaling in these processes.作者: Flustered 時(shí)間: 2025-3-25 04:41
Visualizing Wnt Palmitoylation in Single Cells,gating Wnt activity, secretion, and its interaction with cellular membrane compartments. This protocol describes a method we have recently developed to detect cellular Wnt palmitoylation. The method, combining click chemistry, bio-orthogonal fatty acid probes, and proximity ligation assay (PLA), pro作者: Abbreviate 時(shí)間: 2025-3-25 07:57
Monitoring Wnt Protein Acylation Using an In Vitro Cyclo-Addition Reaction,S-PAGE. This protocol incorporates in vivo labeling of a Wnt-IgG Fc fusion protein using an alkynylated palmitate probe but departs from a traditional approach by incorporating a secondary cycloaddition reaction performed on single-step purified Wnt protein immobilized on protein A resin. This appro作者: Vasoconstrictor 時(shí)間: 2025-3-25 15:28 作者: 集中營 時(shí)間: 2025-3-25 16:07
Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Nototeases to cleave a fragment from the amino terminus of Wnt proteins. Notum is a Wnt deacylase that removes the lipid modification that is essential for Wnt activities. Here, we provide detailed procedures for preparing enzymatic active Tiki and Notum proteins and the in vitro enzymatic reactions. W作者: 驚呼 時(shí)間: 2025-3-25 22:34
Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt Pathway,trol proper signaling output. Recent studies suggest that cell surface Wnt receptor level is controlled by ubiquitination, and serve as a critical regulatory point of Wnt pathway activity as it determines the responsiveness of cells to Wnt signal. Here, we describe flow cytometry, cell surface prote作者: 漸變 時(shí)間: 2025-3-26 00:11 作者: HAWK 時(shí)間: 2025-3-26 04:33 作者: Provenance 時(shí)間: 2025-3-26 09:04
The Use of Chick Embryos to Study Wnt Activity Gradients,troporation can be directed to a single side of the spinal cord, thus leaving the contralateral side as an internal control. Here, we describe a method for measuring Wnt signaling via the use of BAT-Gal, a β-catenin dependent Wnt reporter.作者: 畏縮 時(shí)間: 2025-3-26 13:48
Monitoring Wnt Signaling in Zebrafish Using Fluorescent Biosensors,Our technology is based on artificial enhancers, obtained by polymerization of TCF binding elements, cloned upstream to ubiquitous or tissue-specific promoters. The different promoter/enhancer combinations are used to drive fluorescent protein reporter constructs integrated in the zebrafish germline作者: Gustatory 時(shí)間: 2025-3-26 16:51 作者: TAP 時(shí)間: 2025-3-26 23:33
Reconstitution of the Cytoplasmic Regulation of the Wnt Signaling Pathway Using Xenopus Egg Extractsent in the early embryo of ., and . egg extracts have been used to recapitulate complex biological reactions such as microtubule dynamics, DNA replication, chromatin assembly, and phases of the cell cycle. Herein, we describe a biochemical method for analyzing β-catenin degradation using radiolabel作者: 釘牢 時(shí)間: 2025-3-27 02:35 作者: superfluous 時(shí)間: 2025-3-27 07:31 作者: 強(qiáng)有力 時(shí)間: 2025-3-27 11:24 作者: COKE 時(shí)間: 2025-3-27 16:55 作者: BURSA 時(shí)間: 2025-3-27 17:53
Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells,, (b) a powerful system to model human diseases in a dish, and (c) a platform for examining efficacy and safety of novel drugs. Furthermore, these cells offer a unique opportunity to study early human development in vitro, in particular, the process by which a seemingly uniform cell population inter作者: inhumane 時(shí)間: 2025-3-28 01:15 作者: Anthrp 時(shí)間: 2025-3-28 05:20 作者: 招致 時(shí)間: 2025-3-28 06:59 作者: 小歌劇 時(shí)間: 2025-3-28 12:22 作者: 終端 時(shí)間: 2025-3-28 17:56
Ian J. Huggins,David Brafman,Karl Willerte transformation of these structures. I also describe the seminar ‘with the book in hand’, which is a key activity in the intellectual training of CREA researchers, serving as a space of on-going reflection amid the study of inequalities and our commitment in their transformation through research.作者: 絕種 時(shí)間: 2025-3-28 20:23
requires the development of applications for customers who span multiple regions, languages, currencies, and even customs. If you want to adapt your applications to meet local and native requirements around the world, then this chapter is for you. It will help you understand what internationalizatio作者: foodstuff 時(shí)間: 2025-3-29 01:43 作者: fluffy 時(shí)間: 2025-3-29 05:54 作者: 歸功于 時(shí)間: 2025-3-29 08:49
Annette S. Flozak,Anna P. Lam M.D.,Cara J. Gottardi Ph.D.n Microsoft bought GitHub, things started to evolve and grow; one of the biggest areas of growth was in automation. From that date, Microsoft saw an opportunity to learn from how they evolved their original Azure Pipelines automation and start afresh with those learnings, eventually growing GitHub A作者: 嚴(yán)厲批評(píng) 時(shí)間: 2025-3-29 12:28 作者: –DOX 時(shí)間: 2025-3-29 18:19 作者: 極大痛苦 時(shí)間: 2025-3-29 22:22 作者: PAEAN 時(shí)間: 2025-3-30 00:52 作者: 保守黨 時(shí)間: 2025-3-30 07:42 作者: 屈尊 時(shí)間: 2025-3-30 08:39
Amy T. Ku,Qi Miao,Hoang Nguyene executives from the four customer-facing pillars, delivery, finance, marketing, and channels, can benefit from a new operating model to address the company problem. You will, therefore, need to take action as the first step toward this change by illuminating how each executive will benefit by impr作者: cruise 時(shí)間: 2025-3-30 12:49 作者: 冷淡一切 時(shí)間: 2025-3-30 17:07
Ian J. Huggins,David Brafman,Karl Willerttial academic experience within the ‘feudal’ structures of Spanish academia, I explain how in CREA we have been able to challenge and contribute to the transformation of these structures. I also describe the seminar ‘with the book in hand’, which is a key activity in the intellectual training of CRE作者: 為寵愛 時(shí)間: 2025-3-30 22:45 作者: 血友病 時(shí)間: 2025-3-31 02:08 作者: Popcorn 時(shí)間: 2025-3-31 05:46
s have also shown how data acquisition geometries and signal processing can be combined to create a true Acoustic Seabed Interrogator (ASI), resulting in a set of 4 key recommendations. Chapter 3 describes how this implemented, with the earliest ASI prototype, developed by the lead author between 19作者: 機(jī)制 時(shí)間: 2025-3-31 12:50
978-1-4939-8185-4Springer Science+Business Media New York 2016作者: fixed-joint 時(shí)間: 2025-3-31 14:42 作者: 在前面 時(shí)間: 2025-3-31 21:34 作者: Confirm 時(shí)間: 2025-3-31 22:19
Xinxin Gao,Rami N. HannoushThis chapter explores the rationale, design, and implementation of a Silverlight application that could conceivably exist on the Web. This application features an interactive map, information, images, and video of different parts of a university campus. The finished application is shown in Figure 15-1.作者: LIEN 時(shí)間: 2025-4-1 02:08
Rubina Tuladhar,Nageswari Yarravarapu,Lawrence LumNext up are methods, properties, and fields. Methods define procedures that you can perform on an object or class, while properties enable you to enforce encapsulation by controlling access to the internal state of your objects (fields).作者: EXUDE 時(shí)間: 2025-4-1 09:28
Xiaomo Jiang,Feng CongAim of the study was the identification of possible negative developments in the field of publicly accessible charging infrastructure. The results of the study are summarized again below. In the second part of this section, conclusions for the further development of the legal framework for public charging infrastructure are drawn from the results.作者: prosperity 時(shí)間: 2025-4-1 13:56
Kristin B. Runkle,Eric S. Witze. where we’ll discuss Web-based applications in detail. The first four chapters of the book provided you with a basis for performing updates or creating new applications of any type. The next four chapters discussed desktop application development. In this chapter, we begin looking at the implementation details for Web-based applications.作者: 臆斷 時(shí)間: 2025-4-1 14:44 作者: languid 時(shí)間: 2025-4-1 21:14
Methods in Molecular Biologyhttp://image.papertrans.cn/w/image/1030011.jpg作者: Aggregate 時(shí)間: 2025-4-2 00:32 作者: 夾克怕包裹 時(shí)間: 2025-4-2 04:34
