標題: Titlebook: Whole Genome Amplification; Methods and Protocol Thomas Kroneis Book 2015 Springer Science+Business Media New York 2015 Circulating Tumor C [打印本頁] 作者: 撕成碎片 時間: 2025-3-21 16:59
書目名稱Whole Genome Amplification影響因子(影響力)
書目名稱Whole Genome Amplification影響因子(影響力)學科排名
書目名稱Whole Genome Amplification網(wǎng)絡公開度
書目名稱Whole Genome Amplification網(wǎng)絡公開度學科排名
書目名稱Whole Genome Amplification被引頻次
書目名稱Whole Genome Amplification被引頻次學科排名
書目名稱Whole Genome Amplification年度引用
書目名稱Whole Genome Amplification年度引用學科排名
書目名稱Whole Genome Amplification讀者反饋
書目名稱Whole Genome Amplification讀者反饋學科排名
作者: Femish 時間: 2025-3-21 23:17
Thomas Kroneis,Amin El-Heliebie practice of contestation and argues that, if contestedness is accepted as a meta-organising principle of global governance, regular access to contestation for all involved stakeholders will enhance legitimate governance in the global realm..978-3-642-55234-2978-3-642-55235-9Series ISSN 2191-5466 Series E-ISSN 2191-5474 作者: Interim 時間: 2025-3-22 02:29
Gloria Gonzales Fortes,Johanna L. A. Paijmansts over the past two centuries. From?the isolation and development?of the first alkaloids with morphine that relieved pain within the home and on the battlefield, to the widespread use of nostrums and the addiction crisis that ensued, to the dissemination?of drugs by what became known as Big Pharma 作者: arcane 時間: 2025-3-22 08:01
en the two goals. The success of this readjustment can be seen in the popularity of the confessor-director in the Counter Reformation church. Confession became more frequent since the Fourth Lateran Council had mandated annual confession and less onerous because it included much-desired spiritual di作者: anchor 時間: 2025-3-22 11:14 作者: 北極人 時間: 2025-3-22 15:06
1064-3745 d avoiding known pitfalls..Authoritative and thorough, .Whole Genome Amplification: Methods and Protocols. will serve as a rich source of detailed information and inspiration and 978-1-4939-4846-8978-1-4939-2990-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 作者: 畢業(yè)典禮 時間: 2025-3-22 17:38 作者: 宏偉 時間: 2025-3-23 00:26 作者: SKIFF 時間: 2025-3-23 03:34
Book 2015 necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls..Authoritative and thorough, .Whole Genome Amplification: Methods and Protocols. will serve as a rich source of detailed information and inspiration and 作者: Cpr951 時間: 2025-3-23 08:23
Jeffrey Sabina Ph.D.,John H. Leamon Ph.D.cs include generalized state-space representations, linearization, elimination of prescribed pressures, the tracing of stream lines, lift tables, computational aspects, and the derivation of an energy balance for porous-media flow.作者: exorbitant 時間: 2025-3-23 11:32 作者: DEAF 時間: 2025-3-23 16:19
Zbigniew Tadeusz Czyz,Nikolas H. Stoecklein,Bernhard Polzerational code should greatly facilitate testing some of the theoretical claims of defensive realism versus other grand theories. More importantly, this general “operational code” . guide states’ security strategies today, because the international system has firmly evolved into a defensive realism world (Tang 2009b).作者: Mundane 時間: 2025-3-23 18:52
Bias in Whole Genome Amplification: Causes and Considerations,A. Finally, we attempt to illustrate some of the criteria specific to the analytical platform and research application that should be considered to enable combination of the appropriate WGA method, template DNA, sequencing platform, and intended use for optimal results.作者: Focus-Words 時間: 2025-3-23 22:46
Whole Genome Amplification in Genomic Analysis of Single Circulating Tumor Cells,cular analysis of CTC as liquid biopsy can be used to identify therapeutic targets in personalized medicine directed, e.g. against human epidermal growth factor receptor 2 (HER2) or epidermal growth factor receptor (EGFR) and to stratify the patients to those therapies.作者: SEEK 時間: 2025-3-24 02:46 作者: 厚顏無恥 時間: 2025-3-24 10:03
Low-Volume On-Chip Single-Cell Whole Genome Amplification for Multiple Subsequent Analyses,sferred onto a microliter reaction slide. This slide allows monitoring the single-cell isolation process and isothermal whole genome amplification in less than 2 μL. The amplification products obtained from single cells can be forwarded to multiple analyses.作者: 蓋他為秘密 時間: 2025-3-24 11:18
Detection and Characterization of Circulating Tumor Cells by the CellSearch Approach,ridge for the purpose of downstream single-cell analysis. In this chapter, we will describe in detail the sample acquisition, sample preparation, data acquisition, and assignment of CTC used in the CellSearch system.作者: 不可侵犯 時間: 2025-3-24 17:20 作者: 竊喜 時間: 2025-3-24 22:50
ple and output from it insufficient to carry livelihood sustainably, people suffer from food insecurity. In this chapter, we studied the present status of food security and poverty and suggested how food security can be attained. ?????作者: Antigen 時間: 2025-3-25 02:17
Amin El-Heliebi,Shukun Chen,Thomas Kroneisvival value for groups and that innate affiliative patterns of behaviour have evolved. Relations between members of a group can be seen as an equilibrium between aggression and affiliative processes: the affiliative processes hold the aggressive ones in check. (Argyle, 1970, p. 31)作者: Vasodilation 時間: 2025-3-25 04:30 作者: obsession 時間: 2025-3-25 07:32 作者: 專橫 時間: 2025-3-25 12:39
Quality Control of Isothermal Amplified DNA Based on Short Tandem Repeat Analysis,logenin generating up to 32 different PCR products. After amplification, the PCR products are separated via capillary electrophoresis and analyzed based on the obtained DNA profiles. Isothermal WGA products of good DNA quality will result in DNA profiles with efficiencies of >90 % of the full DNA profile.作者: Conflict 時間: 2025-3-25 18:17
Copy Number Variation Analysis by Array Analysis of Single Cells Following Whole Genome Amplificatiowing single-cell isolation and whole genome amplification. We are focusing on two alternative protocols, an isothermal and a PCR-based whole genome amplification method, followed by either comparative genome hybridization (aCGH) or SNP array analysis, respectively.作者: oxidant 時間: 2025-3-25 21:04 作者: 寬大 時間: 2025-3-26 01:23
Thomas Kroneis,Amin El-Heliebiial role just to have nontrivial Lyapunov inequalities. This fact shows a deep difference with respect to the ordinary case. The linear study is combined with Schauder fixed point theorem to provide new conditions about the existence and uniqueness of solutions for resonant nonlinear problems.作者: JAUNT 時間: 2025-3-26 08:21
Principles of Whole-Genome Amplification,ailable technologies for whole-genome amplification (WGA), bridging the last 25 years from the first developments to currently applied methods. We will especially elaborate on research application, as well as inherent advantages and limitations of various WGA technologies.作者: prosthesis 時間: 2025-3-26 08:30
Sample Preparation Methods Following CellSearch Approach Compatible of Single-Cell Whole-Genome Ampe of pre-enriched samples by means of CellSearch. The techniques described are micromanipulation, FACS, laser capture microdissection, DEPArray, and microfluidic solutions. All platforms are hampered with a low efficiency and differences in hands-on time and costs are the most important drivers for selection of the optimal platform.作者: ARBOR 時間: 2025-3-26 15:08 作者: Contracture 時間: 2025-3-26 20:38
Bias in Whole Genome Amplification: Causes and Considerations, types including individual cells, fossilized remains and entire ecosystems. Multiple methods of WGA have been developed, each with specific strengths and weaknesses, but with a common defect in that each method distorts the initial template DNA during the course of amplification. The type, extent, 作者: 卵石 時間: 2025-3-26 23:21
The Single-Cell Lab or How to Perform Single-Cell Molecular Analysis,le cells. PCR-based amplification techniques are widely used in this field. However, setting up an experiment and analyzing the results can sometimes be challenging. The aim of this chapter is to provide a general overview on single-cell PCR analysis focusing on the potential pitfalls and on the pos作者: 吞下 時間: 2025-3-27 02:50
Sample Preparation Methods Following CellSearch Approach Compatible of Single-Cell Whole-Genome Amp using single cells is to isolate these cells from the surrounding cells, especially when the targeted cells are rare. A number of techniques have been developed for this goal, each having specific limitations and possibilities. In this chapter, five of these techniques are discussed in the light of作者: 發(fā)展 時間: 2025-3-27 06:40
Deterministic Whole-Genome Amplification of Single Cells,e Genomic Hybridization (SCOMP)” (Klein et al., Proc Natl Acad Sci U S A 96(8):4494–4499, 1999). The method has recently become available commercially under the name “.1. WGA Kit.” It is a PCR-based technique for whole genome amplification (WGA) allowing comprehensive and quite uniform amplification作者: 莎草 時間: 2025-3-27 11:20 作者: output 時間: 2025-3-27 14:05
,Heat-Induced Fragmentation and Adapter-Assisted Whole Genome Amplification Using GenomePlex? Singlechnique described here is based on heat-induced random fragmentation yielding DNA strands mainly ranging from 0.1 to 1 kb in length. The fragmented DNA is then subjected to library generation by annealing of adaptor sequences to both ends of the DNA fragments. Using primers hybridizing to the adapte作者: vertebrate 時間: 2025-3-27 21:12 作者: Diverticulitis 時間: 2025-3-28 01:36
Using Multiplex PCR for Assessing the Quality of Whole Genome Amplified DNA,d from heat-induced random fragmented DNA. A set of four primer pairs is used to amplify DNA sequences of WGA products in and downstream of GAPDH gene in yielding 100, 200, 300, and 400 bp fragments. PCR products are analyzed by agarose gel electrophoresis and the respective WGA quality is classifie作者: Panacea 時間: 2025-3-28 05:26 作者: MARS 時間: 2025-3-28 06:20
,Laser Microdissection of FFPE Tissue Areas and Subsequent Whole Genome Amplification by ,1?,ue sections. In this chapter, we describe a workflow for microdissecting small regions of interest from cancer tissue, i.e. formalin-fixed paraffin-embedded (FFPE) and cryo-conserved specimens, and subsequent whole genome amplification by a deterministic WGA approach (.1? WGA).作者: chapel 時間: 2025-3-28 12:51 作者: Dna262 時間: 2025-3-28 17:02
Analysis of Whole Mitogenomes from Ancient Samples,ecies to patterns of domestication. Most of these studies have traditionally been based on the analysis of short fragments from the mitochondrial control region, analyzed using PCR coupled with Sanger sequencing. With the introduction of high-throughput sequencing, as well as new enrichment technolo作者: entitle 時間: 2025-3-28 21:45 作者: MEEK 時間: 2025-3-29 00:15 作者: commute 時間: 2025-3-29 07:01 作者: 出來 時間: 2025-3-29 08:01 作者: MOT 時間: 2025-3-29 13:27 作者: Pessary 時間: 2025-3-29 17:35 作者: transplantation 時間: 2025-3-29 20:26
Joost F. Swennenhuis,Leon Terstappenure, while at the same time endeavouring to find a more efficient system, by introducing the concept of flat-rate benefits, almost regardless of the private use made of the car. There have been changes in the scheme since its introduction and for the sake of simplicity only the current provisions are dealt with in this section.作者: overhaul 時間: 2025-3-30 03:43
,Heat-Induced Fragmentation and Adapter-Assisted Whole Genome Amplification Using GenomePlex? Singler sequences, the DNA is amplified by thermal cycling. This amplification typically yields > 2 mg DNA from a single cell, is suited for amplifying DNA isolated from (partly) degraded samples [e.g. formalin-fixed paraffin-embedded (FFPE) material] and works well when used for array-comparative genome hybridization (array-CGH).作者: wall-stress 時間: 2025-3-30 04:08 作者: 改良 時間: 2025-3-30 08:51
Using Multiplex PCR for Assessing the Quality of Whole Genome Amplified DNA,d according to the number of obtained PCR bands. WGA products that yield three or four PCR bands are considered to be of high quality and yield good results when analyzed by means of array comparative genome hybridization (CGH).作者: 浸軟 時間: 2025-3-30 13:29 作者: 提煉 時間: 2025-3-30 20:28 作者: 毛細血管 時間: 2025-3-30 23:35
Zbigniew Tadeusz Czyz,Stefan Kirsch,Bernhard Polzer viability of rural communities worldwide. Achieving healthy and viable rural communities in the face of rapidly changing social, ecological and economic conditions is a declared global priority. As a result, governments all over the world, in both developed and developing countries, are now priorit作者: sparse 時間: 2025-3-31 00:56 作者: 公共汽車 時間: 2025-3-31 06:10 作者: 割讓 時間: 2025-3-31 09:26
Zbigniew Tadeusz Czy?,Christoph A. Klein to him and his interests at different stages of his life. Those of whom sufficient is deemed to be given incidentally in the Chronology, and well-known authors such as Robert Browning, Thomas Carlyle, George Eliot, and Thackeray, are excluded.)作者: 豐滿有漂亮 時間: 2025-3-31 15:13
Takaaki Kojima,Bo Zhu,Hideo Nakanoic monologue ‘St Simeon Stylites’. The satire is not directed against an individual (Charles Simeon of Cambridge has been suggested) but against the exalted egoism of those who, like St John Rivers in . or Becket in ., act, or are tempted to act, for their own eternal glory. St Simeon is a more extr
